| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,018,373 | 0 | C | G | 52.9% | 14.2 / 24.0 | 17 | A326P (GCA→CCA) | yccS | YccS family putative transporter |
| Reads supporting (aligned to +/- strand): ref base C (1/7); new base G (9/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.11e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.53e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTTCTGATTCAATTGTTGCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTGCGCCGTTATCGCGCATCCGCTCCAGCGCAGCATCAATATGCGTAAAAGCGCGCTCAAAATGCGGATCATGTTGATAAG > NZ_CP009273/1018286‑1018450 | gTTCTGATTCAATTGTTGCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTgcgc < 1:1387629/90‑1 (MQ=255)gTTCTGATTCAATTGTTGCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTgcgc < 1:680112/90‑1 (MQ=255)gTTCTGATTCAATTGTTGCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTgcgc > 1:574031/1‑90 (MQ=255) gATTCAATTGTTGCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTGCGCCGttt > 1:96398/1‑89 (MQ=255) gATTCAATTGTTGCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGGGGGCCGttt > 1:732582/1‑89 (MQ=255) aTTGTTGCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGGGGGCCGTTTTTgcgc > 1:132561/1‑90 (MQ=255) ttgCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTGCGCCGTTATCGCGCATcc < 2:461041/90‑1 (MQ=255) tgCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGGGGGGCGTTATTGCGGATTCg > 2:995753/1‑90 (MQ=255) tgCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGGGGGCCGTTATTGCGCATccc > 1:411666/1‑89 (MQ=255) tgCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGGGGGCCGGTTTTGCGCCTccc > 1:906408/1‑89 (MQ=255) tGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTGCGCCGTTATCGCGCATCCGCTCCAgc < 2:150493/90‑1 (MQ=255) ggCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTGCGCCGTTATCGCGCATCCGCTCCAgcgc < 1:1222855/90‑1 (MQ=255) aaTGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTGCGCCGTTATCGCGCATCCGCTCC‑‑CGCAGCATCaa < 2:1502620/90‑1 (MQ=255) gcgcGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTGCGCCGTTATCGCGCATCCGCTCCAGCGCAGCATCAata < 1:431908/90‑1 (MQ=255) gcgcGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTGCGCCGTTATCGCGCATCCGCTCCAGCGCAGCATCAata < 2:1311162/90‑1 (MQ=255) gcgTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTGGGGCGGTATCCCCCAACCCCTCCCCCGCCGCATCAatata > 2:409158/1‑89 (MQ=255) gCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGGGGGCCGTTTTTGCGCCTCCCCCCCACCGCCGCATCAATAATCGTAAAAacgcgcc > 2:579356/1‑89 (MQ=255) tttGAGTAAATCGGCGGGTGCGCCGTTATCGCGCATCCGCTCCAGCGCAGCATCAATATGCGTAAAAGCGCGCTCAAAATGCGGATCATg < 2:1428528/90‑1 (MQ=255) aaTCGGCGGGGGGGGCGGTTTTGCGCATCCGCTCCCGCCCAGCATCAATATGCGTAAAAGCGCGCTCAAAATGCGGATCATGTTGATAAg > 2:1193402/1‑90 (MQ=255) aaTCGGCGGGGGGGCCGGTTTCGCGCCTCCGCTCCCACGCCGCAACAAAATGCGTTAAAGGGCGCCCAAAATGCGGATCATGGTGATAAg > 1:681915/1‑90 (MQ=255) | GTTCTGATTCAATTGTTGCCAGTTGGGCATCAATGGCGCGTAAATTGTTCAGCAAAAATCCCAGTGTTTTGAGTAAATCGGCGGGTGCGCCGTTATCGCGCATCCGCTCCAGCGCAGCATCAATATGCGTAAAAGCGCGCTCAAAATGCGGATCATGTTGATAAG > NZ_CP009273/1018286‑1018450 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |