| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 52.6% | 18.1 / 28.6 | 19 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/9); new base G (10/0); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152640‑1152816 | cGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTaa < 2:65298/90‑1 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATGCTGGAGATCGGGttt > 2:1396109/1‑90 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATATTGGGTTTCGGGttt > 1:853015/1‑90 (MQ=255) gTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGa < 2:1410812/90‑1 (MQ=255) gTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATAATTGATCTCGGGTTTTTTATTTCCTTTTTCCGCCTTGTGACCTTc > 2:176316/1‑90 (MQ=255) ctacACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGt < 1:1464512/90‑1 (MQ=255) acTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGATCTCGGGTTTTAAATTTCCTTTTTCCGCCTTGTGTCCCTCCCTTATg > 1:700684/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGATCTCGGGTTTTAAATCTCCTTTTTCCGCATTGTGGCCTTCCCTAATGCgg > 1:496321/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATGGATCTCGGGTTTTTTATTTCCTTTTTCGGCATTTTGAGCTTTCCTAATGCgg > 2:445131/1‑90 (MQ=255) ttACAGAGGACAGAGAAACGGCGGGGAAATTACTGGATCTCGGGTTTTAAATCTCCTTTTTCGGCATTTTGACCTTCCGTAAAGCGGGGc > 1:540017/1‑90 (MQ=255) ttACAGAGGACAGAGAAACGGCGGGGAAAATATTGGATTTCGGGTTTTTAATCTCCTTTTTCTGCCTTTTGGGCTTCCCTCATGCGGAgg > 1:764187/1‑89 (MQ=255) ttACAGAGGACAGAGAAACGGCGGGGAAAATAATTGGTCTCGGGTTTTTTAACTCCTTTTTCGGCATTGTGGCCCTCCCTAATTCggggg > 1:847978/1‑87 (MQ=255) cAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTGATGCGGAGCAAc < 2:1582021/90‑1 (MQ=255) cAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAAc < 1:59833/90‑1 (MQ=255) ggACAGAGAAACGGCGGGGAAAATAATTGATTTCGGGTTTTTAATCTCCTTTTTTTGCATTGTGACCTTCCCTAATGCGGAGCAACcgcg > 2:1535603/1‑90 (MQ=255) gACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTgcgc < 1:1588045/90‑1 (MQ=255) agaAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGAtgc < 1:368835/90‑1 (MQ=255) aaCGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGAtgctgc < 2:1424192/90‑1 (MQ=255) cggcggGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTgcgc < 2:737758/90‑1 (MQ=255) ggggAAAATAATGGATCTCGGATTTTTTATCTCCTTTTTCCGCATTGTGAACTTCCGTAATGCGGGGGAACTGCGCGGTGCTGCGCGttt > 2:602538/1‑89 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:1396109/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:363054/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:176316/90‑1 (MQ=255) | CGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152640‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |