| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,213,847 | 0 | G | T | 52.2% | 30.7 / 44.6 | 23 | C355F (TGT→TTT) | pdeG | cyclic diguanylate phosphodiesterase |
| Reads supporting (aligned to +/- strand): ref base G (7/4); new base T (0/12); total (7/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.35e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.04e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCTATCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTT‑CCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTC > NZ_CP009273/1213758‑1213936 | cGCTATCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAgtg > 2:882483/1‑90 (MQ=255) aTCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTa > 2:980546/1‑90 (MQ=255) gTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTT‑cc < 1:288776/90‑1 (MQ=255) ttGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTT‑CCGTGAc < 1:365920/90‑1 (MQ=255) ttGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTT‑CCGTGAc < 2:302476/90‑1 (MQ=255) tttttCATATTGAAATTAATTTTAGCGCTTCGCATTTTTTTCCGCCGACGTTTGTCGACGAGTTTTTAAATTT‑CCGTGACATTTTTcccc < 2:414514/89‑1 (MQ=255) tGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTT‑CCGTGACAGTTTTACCCGCCGCGAttt > 2:1308201/1‑90 (MQ=255) attTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTTTTTAAATTT‑CCGTGACAGTTTTACCCGCCGCGATTTAAACCTTg < 1:271601/90‑1 (MQ=255) cTTCGCATTTTTTTCCCCCGACGTTTGTCGACGAGTTTTTAAATTT‑CCGTGACAGTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAg < 2:437633/90‑1 (MQ=255) tCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTT‑CCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTc > 1:628582/1‑90 (MQ=255) cATTTTTTTCCGCCGGCTTTTGTCGACGGGTTTTTAATTTT‑CCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCAcc < 2:406272/90‑1 (MQ=255) cATTTTTTTCCGCCGATTTTTGTCGACGAGTTTTTAATTTT‑CCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCAcc < 1:732916/90‑1 (MQ=255) atttttattCCGCCGACTTTTGCCGAGGA‑TTTTTAAATTTCCCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCAcc < 1:1234409/86‑1 (MQ=255) tttCGCCGACGTTTGTCGACGAGTGTTTAAATTT‑CCGTGACAGTTTTACCAGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCg > 2:663452/1‑88 (MQ=255) tttCGCCGACGTTTGTCGACGAGTGTTTAAATTT‑CCGTGACAGTTTTACCAGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCg < 1:663452/88‑1 (MQ=255) ttCGCCGCCTTTTGTCGAGGAGTTTTTAAATTC‑CCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGa < 2:1091119/90‑1 (MQ=255) tCGCCGACGTTTGTCGACGAGTGTTTAAATTT‑CCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAg > 1:520537/1‑90 (MQ=255) cGCCGTGGTTTGTCGAGGAGTTTTTAAATTT‑CCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGc < 2:1397937/90‑1 (MQ=255) gACGTTTGTCGACGAGTGTTTAAATTT‑CCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCAtt > 1:682879/1‑90 (MQ=255) ttttGTCGACGAGTTTTTAAATTC‑CCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGaa < 1:1136196/89‑1 (MQ=255) ttGTCGAGGAGTTTTAAATTTT‑CCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATg < 1:882483/90‑1 (MQ=255) gaGTTTTTAATTTT‑CCGTGACATTTTTCCCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGaa < 2:520537/90‑1 (MQ=255) gaGTTTTTAAATTT‑CCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGa < 1:459701/71‑1 (MQ=255) gaGTGTTTAAATTT‑CCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGa > 2:459701/1‑71 (MQ=255) gtTTAAATTT‑CCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTc > 1:1232992/1‑90 (MQ=255) | CGCTATCGCGAGTAAATTGCCGGAAGGTTTTCATATTGGAATTAATTTTAGCGCTTCGCATATTATTTCGCCGACGTTTGTCGACGAGTGTTTAAATTT‑CCGTGACAGTTTTACCCGCCGCGATTTAAACCTTGTTCTGGAAGTCACCGAGCGTGAGCCATTGAATGTTGATGAAAGTC > NZ_CP009273/1213758‑1213936 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |