| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,350,458 | 0 | T | G | 53.8% | 9.8 / 17.2 | 13 | T304P (ACC→CCC) | sapA | peptide ABC transporter substrate‑binding protein SapA |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (7/0); total (7/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.83e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTG > NZ_CP009273/1350369‑1350543 | gtaataGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGgt < 1:1314341/89‑1 (MQ=255) aGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGttta > 1:1193489/1‑88 (MQ=255) tGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGG‑TTTGGGGGGGTTaatg > 2:563478/1‑88 (MQ=255) gATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GGTTTTGGGGGGGTTAAAAGc > 1:331035/1‑90 (MQ=255) gATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGcc < 1:257270/90‑1 (MQ=255) gATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑GTTAGCGGGGTTTAATGcc > 2:1234429/1‑90 (MQ=255) aGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCg < 1:295511/90‑1 (MQ=255) tGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACGTTc < 2:180787/90‑1 (MQ=255) attaATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGCGGG‑TTTGGGGGGGTTAAAT‑CCCGAAAAGCGGCGGTCATccc > 2:510681/1‑90 (MQ=255) gCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGc < 1:563478/90‑1 (MQ=255) tGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGGGTTAAATGCCAGATAGGCGACGTTCATCCCAGGGCGCAGCGTTAAAAGCAAg > 1:421383/1‑90 (MQ=255) cGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAgcgc < 1:494629/90‑1 (MQ=255) gg‑TTTGGCGGGGTTAAATGCCAGATAGGCGACGTTCATCCCCGGACGCAGCGTTAAAAGCAAGCGCGGGGCGTCACGCAAAAAGGATaac > 2:560904/1‑88 (MQ=255) gCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAgctggctg < 1:952166/90‑1 (MQ=255) | ATAATAGATGGATTGCATCAGGCGCTGGTTATTAATCGCCAGTGCCAGCGCATGGCGGACAGCGGGATTATTTAGCGGC‑GG‑TTTGGCGGTGTTAAATGCCAGATAGGCGACGTTCATCCCAGGACGCAGCGTTAAACGCAAGCGCGGGTCGTCACGCAAAATGGATAGCTGGCTG > NZ_CP009273/1350369‑1350543 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |