| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,778,686 | 0 | T | G | 52.5% | 72.3 / 90.9 | 59 | intergenic (‑316/+435) | BW25113_RS25705/BW25113_RS13825 | DUF5507 domain‑containing protein/tRNA‑Ile |
| Reads supporting (aligned to +/- strand): ref base T (8/20); new base G (31/0); total (39/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.11e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.50e-11 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTAC > NZ_CP009273/2778606‑2778774 | aaTTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTc < 1:1167519/90‑1 (MQ=255)aaTTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTc < 2:367641/90‑1 (MQ=255)aaTTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTc < 1:372649/90‑1 (MQ=255) cagcAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGtagtag < 1:806577/87‑1 (MQ=255) gTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTTGGCAGTAGTAGATGTTTAAgg > 2:248576/1‑90 (MQ=255) gTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGGCAGTAGTAGATGttttaag > 1:1225395/1‑86 (MQ=255) gTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGGTTTGGGCAGTAGTAGATGTTTAAgg > 2:54417/1‑90 (MQ=255) gTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGGTCTGGTCAGTAGTAGATGTTTTAgg > 1:245156/1‑90 (MQ=255) ggTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGGCAGTAGTAGATGTTTTAGGCgg > 2:81599/1‑89 (MQ=255) ggTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTTAGGCgg > 1:1355716/1‑89 (MQ=255) ggTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGGTCTGGGCAGTAGGAGATGTTTAAGGCgg > 1:808636/1‑89 (MQ=255) caggAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCa < 1:219080/87‑1 (MQ=255) cATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGAt < 2:204877/69‑1 (MQ=255) cATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGAt > 1:204877/1‑69 (MQ=255) aCGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTTTGGTCAGTAGGAGATGTTTTAGGGGTGGCAGAGACATTTTATc > 1:674935/1‑90 (MQ=255) aCGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTc < 2:219258/52‑1 (MQ=255) aCGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTc > 1:219258/1‑52 (MQ=255) aCGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACAtt > 1:534955/1‑85 (MQ=255) aCGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACAtt < 2:534955/85‑1 (MQ=255) aCGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGGCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATc > 1:1315314/1‑90 (MQ=255) aCGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTTGTCAGTAGTAGATGTTTAAAGCGGGGGCGAGACAatttctg > 2:219080/1‑86 (MQ=255) aCGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGGTCTGGTCAGTAGTAGATGTTTTAGGGGGGGCAGAGACATTTTATc > 2:421908/1‑90 (MQ=255) gCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCCGTAGTAGATGTTTTAGGCGTGGCAGAGACATTTCATCCt > 1:199379/1‑90 (MQ=255) gCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGGTGGAAGGAGATGTTTAAAGGCTGGGAGAGACATTTCATCCt > 1:1307535/1‑90 (MQ=255) gCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGGTTTGGTCGGTAGTAGGAGTTTTAAGCGTGGCAGAGACATTTTATCCt > 1:1082157/1‑90 (MQ=255) gCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGGGGTTTGGTCAGGAGTAGAGGTTTAAAGCGTGGCAGAGACATTTCATCCt > 2:1373797/1‑90 (MQ=255) cTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCtt < 1:845139/90‑1 (MQ=255) ccTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTAc < 2:873189/90‑1 (MQ=255) ccTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTAc < 1:627131/90‑1 (MQ=255) ccTGTAACGCTCACAAAACTCATCTGCCCGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTAc < 2:1050982/90‑1 (MQ=255) aCGCTCACAAAACTCATCTGCCTGCGGCGGGGGGTCTGGTCAGTAGTAGATGTTTAAGGCGGGGCAGAGACATTTTATCCCTTCTCTACg > 2:578380/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCa > 1:710810/1‑40 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCa < 2:710810/40‑1 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTTGTCAGTAGTAGATGTTTTAGGCGGGGGAGAGACATTTCATCCTTTATCTTCGGc > 2:1075754/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGTCAGTAGTAGATGTTTAAGGGGGGGCGGAGACATTTTATCCTTTCTCTACGGc > 2:1297883/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGGCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTTATCCTTACTCTACGGc > 2:607393/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTTAGGCGTGGGAGAAACATTTCATCCTTACTCTTCGGc > 1:670191/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTTAGGCGGGGGCGAGACATTTTTTCCTTTATCTACGGc > 1:1417633/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGc > 1:636107/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGc > 1:309909/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTAAGGCGGGGCAGAGACATTTCATCCTTACTCTACGGc > 1:1077101/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTAAAGCGGGGGAGAGACATTTTATCCTTTCTCTACGGc > 2:627131/1‑90 (MQ=255) gCTCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGGCAGTAGTAGATGTTTAAGGCGGGGGAGAGAAATTTCATCCTTTCTCTACGGc > 1:1088626/1‑90 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCa < 1:1010511/38‑1 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCa > 2:1010511/1‑38 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTTAGGCGGGGGAGAGACATTTTATCCTTTCTCTACGGCAt > 1:1297731/1‑90 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCAt > 1:884147/1‑90 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCAt < 1:421908/90‑1 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGGGTTTTGGTCAGTAGTAGATGTTTAAAGGGGGGGAGAGACATTTTATCCTTTCTCTACGGCAt > 1:1273616/1‑90 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGGGTTCTTGTCAGTAGTAGATGTTTAAGGCGGGGCAGAGACATTTTATCCTTTCTCTACGGCAt > 2:536519/1‑90 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTTAAGCGGGGCAGAGACATTTTATCCTTTCTCTACGGCAt > 2:167620/1‑90 (MQ=255) tCACAAAACTCATCTGCCTGCGGCGGGGGTTCTGGGCAGGAGTAGATGTTTAAGGCGGGGCAGAGACATTTCATCCTTTCTCTACGGCAt > 1:759447/1‑90 (MQ=255) cacaAAACTCATCTGCCTGCGGCGGGGGTTCTGGGCAGTAGTAGATGTTTAAGGCGGGGCAGAGACATTTCATCCTTACTCTACGGCAtt > 1:1339488/1‑90 (MQ=255) tCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCtaca < 2:757667/90‑1 (MQ=255) tCATCTGCCTGCGGCGGGGGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTTATCCTTTCTCTACGGCATTGTTCtaca > 2:423360/1‑90 (MQ=255) cATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCtacat < 1:536519/90‑1 (MQ=255) tgcctgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTacatacat < 2:884147/90‑1 (MQ=255) tgcGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGt < 2:144285/90‑1 (MQ=255) cggcggGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTtg < 1:914861/90‑1 (MQ=255) gtgtTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTAc < 1:167620/90‑1 (MQ=255) gtgtTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTAc < 1:1209486/90‑1 (MQ=255) gtgtTCCGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGGCATTTCATCCTTTCTCTACGGCATTGTTCTACATACATTGGTTGTGGttc < 2:821712/90‑3 (MQ=255) | AATTTATATCTCAATAGCGTTGGTTAATGAGCATAGCCACGCTCCTGTAACGCTCACAAAACTCATCTGCCTGCGGCGGGTGTTCTGGTCAGTAGTAGATGTTTAAGGCGTGGCAGAGACATTTCATCCTTACTCTACGGCATTGTTCTACATACATTGGTTGTGGTAC > NZ_CP009273/2778606‑2778774 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |