| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 49,791 | G→A | intergenic (+160/‑32) | kefC → / → folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 49,791 | 0 | G | A | 98.4% | 235.2 / ‑4.1 | 63 | intergenic (+160/‑32) | kefC/folA | glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base G (0/0); major base A (37/25); minor base T (1/0); total (38/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-01 | |||||||||||
GAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAC > NZ_CP009273/49706‑49876 | gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGc > 2:789289/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCg > 1:1044089/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCg > 1:307531/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCg > 1:1541151/1‑90 (MQ=255) agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCg > 2:556370/1‑90 (MQ=255) gtgtACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAAt > 2:1261503/1‑90 (MQ=255) gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAAt > 2:853016/1‑90 (MQ=255) ggACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAAttt > 2:125494/1‑90 (MQ=255) ggACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAAttt > 2:1174315/1‑90 (MQ=255) ggACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAAttt > 1:311024/1‑90 (MQ=255) cGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAAtttttttt > 2:614138/1‑90 (MQ=255) gcagAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCgg > 1:421552/1‑90 (MQ=255) gAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGaa < 1:556370/90‑1 (MQ=255) gAATATAAAAGTTGCCACAA‑CAGCAACCTCGCTCCAGTCGCCGACGGTTTACGCTTTACGCAT‑AATGGCGATAATTTTTTTTATCGGGaa < 2:726655/90‑1 (MQ=255) atatAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAAt < 2:307531/90‑1 (MQ=255) atatAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAAt < 2:1123464/90‑1 (MQ=255) atatAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAAt < 1:84889/90‑1 (MQ=255) atatAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAAt > 1:114658/1‑90 (MQ=255) ataAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAAtct < 1:1139708/90‑1 (MQ=255) ttCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc < 1:215737/90‑1 (MQ=255) ttCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc > 2:775525/1‑90 (MQ=255) ttCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc > 2:560264/1‑90 (MQ=255) ttCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc < 2:311024/90‑1 (MQ=255) ttCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc < 1:1264818/90‑1 (MQ=255) ttCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc > 2:1264818/1‑90 (MQ=255) ttCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc < 1:809145/90‑1 (MQ=255) ttCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc < 1:474376/90‑1 (MQ=255) ttCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc > 1:554211/1‑90 (MQ=255) ttCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTGACGCTTTACGTAT‑AATGGCGACAATTTTTTTTACCGGGAAATCTCAATGATc > 2:986587/1‑90 (MQ=255) ttCCCCAACCATCATCCTCGCCCCAGTCGCCGCCGGTTTCCGCTTTACG‑AT‑AAAGGGGGCCAATTTTTTTATCGGGAAATCTCAATGATc < 1:1227934/90‑1 (MQ=255) ccTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAg > 1:387847/1‑90 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATg < 1:511273/70‑1 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATg > 2:511273/1‑70 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt > 1:604685/1‑90 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt > 1:791407/1‑90 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt < 2:1155985/90‑1 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt < 2:604685/90‑1 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt < 2:566144/90‑1 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt > 1:971668/1‑90 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt < 2:1439228/90‑1 (MQ=255) cGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt < 2:387847/90‑1 (MQ=255) ccAGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATCTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCg < 1:1582241/90‑1 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTATAATTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCgg > 1:1486297/1‑90 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGTGGCGTTTGCGGt > 2:896915/1‑90 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 2:375126/1‑90 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 1:1592463/1‑90 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 1:811312/1‑90 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 1:1337542/1‑90 (MQ=255) aGTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGt > 1:1424053/1‑90 (MQ=255) gTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCa > 2:1072544/1‑60 (MQ=255) gTCGACGACGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCa < 1:1072544/60‑1 (MQ=255) gacgacGGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGAt > 1:248149/1‑90 (MQ=255) acgacgGTTTACGCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATc < 2:919174/90‑1 (MQ=255) gCTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCa < 2:791407/90‑1 (MQ=255) cTTTACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCAt < 2:114658/90‑1 (MQ=255) tttACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATg > 2:1450285/1‑90 (MQ=255) ttACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATgg > 1:1460598/1‑90 (MQ=255) ttACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATgg > 2:964058/1‑90 (MQ=255) tACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGa > 2:773007/1‑90 (MQ=255) tACGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGa > 1:545708/1‑90 (MQ=255) aCGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGaa < 1:1174315/90‑1 (MQ=255) aCGTAT‑AATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGaa < 1:595698/90‑1 (MQ=255) tat‑aATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAc > 1:1003494/1‑90 (MQ=255) | GAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAA‑CATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTAT‑AGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAAC > NZ_CP009273/49706‑49876 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |