| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 822,410 | 0 | A | T | 56.2% | 4.6 / 21.0 | 16 | I100N (ATT→AAT) | ybhS | ABC transporter permease |
| Reads supporting (aligned to +/- strand): ref base A (7/0); new base T (0/9); total (7/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTG > NZ_CP009273/822332‑822495 | aTCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGcc > 2:419984/1‑90 (MQ=255) ggtgcggtgGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCg > 2:543431/1‑90 (MQ=255) ggggggCGTGGGCGCGCCCCTTTTGTCCCGAAAAACCCCCCGAAAAACCCCCCAGCCCGCAATTTTCCCCCGCCTGCATTTTGGCGATCa < 2:23566/86‑1 (MQ=255) ggtgGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAg > 1:653335/1‑90 (MQ=255) gggggCGTGGCCGCGCCCCTTCTTTCCCGAAAAACCCCCCGAAAAACCCCCAAGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATCAg < 1:543431/87‑1 (MQ=255) gtgGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGt > 1:47845/1‑90 (MQ=255) tGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGAtg > 1:714797/1‑90 (MQ=255) aaaaCCCCCCGAAATACCCCCCAGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTc < 1:419984/90‑1 (MQ=255) acccaccaGCCCGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAAcc < 2:576881/88‑1 (MQ=255) aaCCACCAGACCGAGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAAcc < 2:629811/90‑1 (MQ=255) caGCCCGCAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCAt < 1:673798/90‑1 (MQ=255) caGCCCGCAATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCAt < 2:653335/90‑1 (MQ=255) caGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCAt > 1:341155/1‑90 (MQ=255) ccGCTATTTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGgtg < 2:397629/90‑1 (MQ=255) ccGCGATTTTCCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGgtg < 2:244692/90‑1 (MQ=255) gcgAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGtgtg > 2:666881/1‑90 (MQ=255) | ATCACCTGAATCGGTGCGGTGGCGTTGGCGCGCTCCATCTGTTCCGCAAAATCCACCGGAATAACCACCAGACCGCGAATTTTCCCCGCCTGCATTTTGGCGATCAGTTCCTGACGGTTATCGCTGATGGTGGCGTCGATGTAGGGCGAACCGGTCATGGTGTG > NZ_CP009273/822332‑822495 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |