| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 832,048 | 0 | T | G | 64.7% | 6.0 / 14.6 | 17 | V81G (GTC→GGC) | hcxB | hydroxycarboxylate dehydrogenase HcXB |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (11/0); total (11/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACG > NZ_CP009273/831962‑832131 | gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCAc > 1:472978/1‑90 (MQ=255) tGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCTCGATGGCGa > 1:185816/1‑90 (MQ=255) ccTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCAt < 1:302457/90‑1 (MQ=255) ggAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGGAACCCCGATGGGGAACGCGCCtttt > 2:195107/1‑89 (MQ=255) gTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTc < 2:441327/90‑1 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCGCGCCCGCAGGGGAGCGCGCATTTTGGCAg > 2:419626/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCCCCCCCCCTGGCGAACGCGCATTTTGGCAg > 2:302338/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCCCCCCCCATGGCGGGCGCGCCTTTTGGCAg > 1:625872/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCTCGATGGCGATCGCGCATTTGGTCAg > 1:31277/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACGCCCCATGGGGGACGCGCCTTTTGTCAg > 2:151170/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACCCTCCTTTGGGACCGCGCATTTGGCCAg > 1:10720/1‑90 (MQ=255) tGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCt < 1:572726/90‑1 (MQ=255) acaggTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGAt < 1:344228/86‑1 (MQ=255) gTTAAAGAGGCGGGGGGGGCGGGCACGCTCGGTGGGGATCGCGCATTTTGTCAGGGCGCGGGCCATGAAGCGATGGGGGTGGGGGGTgag > 2:89697/1‑90 (MQ=255) gTTAAAGAGGCGGGGGGGGCGGGCACGCCCGATGGCGATCGCGCATTTTGTCAGGGCGCGGGACATGAAACGATGGGGCTGGGGGTTggg > 2:313599/1‑88 (MQ=255) gcggcgGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAc < 1:551586/90‑1 (MQ=255) cggcggTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACg < 2:472978/90‑1 (MQ=255) | GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCGCATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACG > NZ_CP009273/831962‑832131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |