| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,126 | 0 | T | C | 58.8% | 10.2 / 16.4 | 17 | I425T (ATC→ACC) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (6/1); new base C (0/10); total (6/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.66e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.36e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGC > NZ_CP009273/3214062‑3214209 | tttCCCAGCGAGATGTCCCGCCCGCCCGTACGGGGGCCCGAAC‑CGCTCAAAACCGCAAAACCGACCCGCATTAACCCGCcactgacactg < 2:9762/90‑1 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 1:592931/1‑90 (MQ=255) cAGGGAGTTGTCCCGCCGGCGGGTACGGGGGGCCGGAC‑CGCTAAAAAACGCAAAAACGACCCGTATTGAACCGCCACTGACACTGACCAt < 1:646062/90‑1 (MQ=255) gagaTTTCCCGCCAGCGCGTACGGGGGGCCGGAA‑CGCTAAAAAACGCAAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGaa < 1:276323/90‑1 (MQ=255) agttGTCCCGCCAGCGCGTCCTGGTGGCCGGAA‑CCCTCAATAACCCAAAAACGACCCGCATTAAACCGCCACTGACACTGACCATTGAAc < 1:17368/87‑1 (MQ=255) agaTTTCCCCCCGGCGCGTCCGGGTGGCCGGAC‑CCCAAAAAACCCCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:508904/90‑1 (MQ=255) agaTGTCCCGCCGGCGCGTCCTGGGGGCCGGAA‑CCCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 1:19623/90‑1 (MQ=255) aTGTCCCGCCGGGGCGT‑CGGGGGGCCCGAACCGCTAAAAAACCCAAAACCGCCCCGCATTAACCCGCCACTGACACTGACCATTGAACAg < 2:592931/90‑1 (MQ=255) aTGTCCCGCCAGCGCGTACTGGGGGCCGGAA‑CGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAg < 1:160547/90‑1 (MQ=255) gCCAGCGCGTGCTGGTGGCGGGAA‑CGCTAAATAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAAc < 1:183883/90‑1 (MQ=255) ggcgcGTCCGGGGGGCGGGAC‑CGCCAAAAACCGCAAAAACGACCCGCATTAACCCGCCACTGACACTGACCATTGAACAGTGTGAACTgg < 1:377262/89‑1 (MQ=255) ggCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcggc > 2:107468/1‑90 (MQ=255) gAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTa > 2:235061/1‑90 (MQ=255) gAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGAGATCAAAGCGGGGCGTa > 2:58818/1‑90 (MQ=255) a‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAg > 1:429301/1‑90 (MQ=255) aaaaCGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATg > 1:182472/1‑90 (MQ=255) aaccGACCCGCATAAACCCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGc < 2:182472/87‑1 (MQ=255) | TTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAA‑CGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGC > NZ_CP009273/3214062‑3214209 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |