| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,515,736 | 0 | T | G | 57.1% | 8.3 / 13.8 | 14 | S125S (TCA→TCC) | hofM | DNA utilization protein HofM |
| Reads supporting (aligned to +/- strand): ref base T (1/5); new base G (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.25e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCGGCAGTCACGTTATAGGCGGGACTGAGTGAGTCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGT > NZ_CP009273/3515647‑3515816 | cTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCGGCAGTCACGTTATAGGCGGGACtgagt < 2:571775/90‑1 (MQ=255) cacaTGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCGGCAGTCACGTTATAGGCGGGACtgagtga < 2:187022/90‑1 (MQ=255) acaTGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCGGCAGTCACGTTATAGGCGGGGCtgagtgag > 1:262385/1‑90 (MQ=255) cGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCGGCAGTCACGTTATAGGCGGGGCTGAGGGAGTCTTccccg > 2:147452/1‑86 (MQ=255) tCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCGGCAGTCACGTTATAGGCGGGACTGAGTGAGTCTTCGCTGTAATCGAAg < 2:687107/90‑1 (MQ=255) tCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCGGCAGTCACGTTATAGGCGGGACTGAGTGAGTCTTCGCTGTAATCGAAg < 2:539962/90‑1 (MQ=255) tGCCCGACTAAGCCGCGTTGCCAGCACTTTCCTTTGCGAGGCAGCCACGCTATAGGCGGGACTGAGTTAGTCTTCGCTGTAATCGAAgcg < 2:605157/90‑1 (MQ=255) agagTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCGGCAGTCACGTTATAGGCGGGGCTGAGGGAGGCTTTTCTGTAATCGAAGCGCAgg > 2:262304/1‑90 (MQ=255) gTTGCCAGCTCTTTGCTTTGCGCGGCAGTCACGTTATAGGCGGGACTGAGGGGGTCCTTGCTGTTATTGAAGCGCAGGGAGGCCgggtac > 2:527131/1‑86 (MQ=255) ttgctttgcGCGGCAGTCACGTTATAGGCGGGACTGGGGGAGTCTTCTCTGTTATCGAAACGCAGGGGGGCCGGGTCCATATCCAGCTcg > 2:621435/1‑90 (MQ=255) ttgctttgcGCGGCAGTCACGTTATAGGCGGGACTGAGGGAGTCTTCGCTGTAATCGAAACGCAGGGAGGCCGGGTCCATATCCAACTcc > 2:231093/1‑89 (MQ=255) ctttgcGCGGCAGTCACGTTATAGGCGGGACTGAGTGAGTCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCgg < 1:348395/90‑1 (MQ=255) gCAGTCACGTTATAGGCGGGACTGAGTGAGTCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTc < 1:262304/90‑1 (MQ=255) gCAGTCACGTTATAGGCGGGACTGAGGGAGTCTTCCCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCCGCTCGCGGGCCATCGTc > 1:225492/1‑90 (MQ=255) gTCACGTTATAGGCGGGGCTGAGGGGGGCTTCGCTGTAATCGAAGCGCAGGGGGGCCGGGTCCATTTTCCGCTCGCGGGCCATCGGCCCt > 1:596498/1‑90 (MQ=255) tataGGCGGGACTGAGGGAGTCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGcc > 1:518630/1‑90 (MQ=255) gggACTGAGTGAGTCTTCGCTGTAATCGAAGCGCAGGGAGGCCGGATCCATATCCAGCTCCCGGGCCCACGTCCCCGACAACCAGGCCgg > 2:645676/1‑89 (MQ=255) | CTCACATGAACACGCAACCTTTCTGCCAGAGTAAGCAGCGTTGCCAGCTCTTTGCTTTGCGCGGCAGTCACGTTATAGGCGGGACTGAGTGAGTCTTCGCTGTAATCGAAGCGCAGGGAGTCCGGATCCATATCCAGCTCGCGGGCCATCGTCCCTGACAGCCAGGCCGT > NZ_CP009273/3515647‑3515816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |