| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 263,428 | 0 | G | T | 54.5% | 0.4 / 11.0 | 11 | Q97K (CAG→AAG) | yafZ | DUF932 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (3/2); new base T (0/6); total (3/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.06e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.40e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATTTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTG > NZ_CP009273/263372‑263512 | cATCTGATCCGAACTGTTTCCATCGGGAGAGTTGAGTAGAATATTTTCCGGCACCTTTTTACGGGTGATCTGCCCTTCCCGCCGCAGACg < 1:436440/90‑1 (MQ=255) aCGATCTGTTACCACCGTGAGGGTAGATTAAAATAATTCCCGGCCCCTGTTTACGGGTGATCTGCCTTCCCCGCCGCAGACGCAGCatat < 1:417878/90‑1 (MQ=255) gAACTGTTCCAATCGGGAGAGTTGAGTAAAAAAATTCCCGGCCCCTTTTTCCGGGTGATCTCCCCTTCCCGCCGCAGACGCAGCATATGc < 2:271323/90‑1 (MQ=255) gAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATTTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGc > 2:668407/1‑90 (MQ=255) cATCGTGAGAGTTGAGTAAAATATTTCCCGGCCCTTTTTTACGGGTGATCTCCCCTCCCCGCCGCAGACGCAGCATATGCTTTGTATGtt < 2:29895/90‑1 (MQ=25) ggagaGTGGAGTAAAATATTTTCCGGCCCTTTTTTCCGGGTGATCTCCCCTCCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCacga < 2:427703/89‑1 (MQ=255) gagaGTTGAGTAAAATAATTCCCGCCCCCTTTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCacgac < 1:229521/90‑1 (MQ=37) taaaatattTCCCGGCCCCTGTTTCCCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTc < 1:580584/87‑1 (MQ=25) gAATAATTTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTcac > 1:356150/1‑90 (MQ=255) gCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCt > 1:740683/1‑90 (MQ=255) ccccTTTTTCCGGGTGTTCTCCCCTCCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTg < 2:356150/88‑1 (MQ=37) | CATCTGATACGAACTGGTTCCATCGTGAGAGTTGAGTAGAATAATTTCCGGCACCTGTTTACCGGTGATCTGCCCTTCCCGCCGCAGACGCAGCATATGCTTTGTATGTTCACGACGACCCGGGTCACGCACGCGGGTCTG > NZ_CP009273/263372‑263512 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |