Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP009273822,9550TG66.7% 3.4 / 12.6 15T495P (ACC→CCC) ybhFATP‑binding cassette domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/5);  new base G (10/0);  total (10/5)
Fisher's exact test for biased strand distribution p-value = 3.33e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCC  >  NZ_CP009273/822881‑823025
                                                                          |                                                                      
aaaGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGcc                                                         <  1:189235/90‑1 (MQ=255)
     gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAg                                                    >  2:223747/1‑90 (MQ=255)
     gggtggTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGaaa                                                    >  2:711097/1‑89 (MQ=255)
            gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACCCCGGGAGTCGGGtc                                             >  1:745367/1‑90 (MQ=255)
            gACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGGCGGTtc                                             >  1:238464/1‑90 (MQ=255)
                      gTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGGAACCCCGGAAGACGGGTCGTCGGGaaaa                                   >  2:491453/1‑89 (MQ=255)
                          cGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGaa                               <  1:813369/90‑1 (MQ=255)
                              tttCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGGAGTGGGGTCGTCGAGAAACAGAATGtg                           >  2:314264/1‑89 (MQ=255)
                                    tACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAAAGTCCGGGTc                     >  2:29977/1‑90 (MQ=255)
                                        aTGCTGTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCGGAAGTCGGTTCGTCGAGAAAAAGAAAGTCCGGGTCATGc                 >  2:339921/1‑90 (MQ=255)
                                             gTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAg            <  1:339921/90‑1 (MQ=255)
                                             gTTGATGTGCAGCCAAAATTCACGGCGGGGGGGGGGGGCAAAGCCGGGAGGCGGGTCGTCGGGAAAAAAAAAGGCCGGGTCCTGCATCaa            >  1:827887/1‑89 (MQ=255)
                                                  tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc       <  2:383657/90‑1 (MQ=255)
                                                  tgtgCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAAc       <  2:577651/90‑1 (MQ=255)
                                                       aGCCAAAATTCACGGCGGGGGGGGGGGGCAACGCCCGGAGGCGGGTTGTCGGGAAACAGAAAGTCCGGGTTATGCATCAGCGGACAGgcc  >  1:383657/1‑90 (MQ=255)
                                                                          |                                                                      
AAAGTGGGTGGTGACCATCACCGTGACGCCTTTCTCTACCATGCTGTTGATGTGCAGCCAAAATTCACGGCGGGTGAGGGGGTCAACGCCGGAAGTCGGTTCGTCGAGAAACAGAATGTCCGGTTCATGCATCAGCGAACAGGCC  >  NZ_CP009273/822881‑823025

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑