| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,476,148 | 0 | T | C | 55.6% | 7.3 / 18.9 | 18 | F336S (TTC→TCC) | ynbD | phosphatase PAP2/dual specificity phosphatase family protein |
| Reads supporting (aligned to +/- strand): ref base T (7/1); new base C (0/10); total (7/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.51e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.11e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGACTGCTGGTGTTTATTTAGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGC > NZ_CP009273/1476070‑1476228 | tGACTGCGGGTTTTTATTTGGGGGCTTTCCCACGACATTTCCCGGCAAAAAATGCGGTTCTGGACGTCCCTTTTGAATTCCCTCGCGGAc < 1:367140/90‑1 (MQ=255) cctgctgTTTTTTTTTTGGGGGCTTTTCCACGACATTTCCCGGCAAAGAATGCGGTTCTGGACGTCCCTTTTGATTCCCCTCGCGGACGa < 1:303241/89‑1 (MQ=255) gtTTATTTAGGGGCGTCTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACaaaa > 2:201509/1‑90 (MQ=255) tgggggCTTTTCCACGACATATCCCGGCACAGAATGCGGTTCTGGCCGTCCCTTTTGAATCCCCTCGCGGACGAGCCACAAAAGATCGAc < 1:187244/88‑1 (MQ=255) tgggggCTTTCCCACGACATATTCCGGCAAAAAATGGGGTTCTGGACTTCCCTTTTAAATCCCCCGGGGGAGGAGCCACAAAAGATCGAc < 1:423963/88‑1 (MQ=255) gggggCTTTCCCACGACATTTCCCGGCACAGAAGGGGTTTCTGGCCGCCCCCTTTAATTCCCCCCGCGGACGAGCCACAAAAGATCGAct < 1:145713/89‑1 (MQ=255) aGGGGTGTTTCCACGACATATTCCGCAAAAGAATGCGGTTTTGGACGTCACCTTTAAATCCCCCCGGGGCAGAGCCACAAAAGATCGAct < 2:167056/90‑1 (MQ=255) aGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGAct > 1:517112/1‑90 (MQ=255) gggCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGActct > 1:662725/1‑90 (MQ=255) gTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTAttt > 2:423963/1‑90 (MQ=255) atTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATg > 1:778318/1‑90 (MQ=255) cccGGCAAAGAATGGGTTTCTGGCCGTCCCCTTTGATTCCCCCCGGGGAGGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCt < 1:201509/89‑1 (MQ=255) gggCACAGACTGCGGTTCTGGCCTTCCCTTTTCAATCCCCCCGGGGAGGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTgg < 1:765869/89‑1 (MQ=255) aaggCGGTTCGGGCCTTCCCCTTTAAATCCCCCCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCtggtg < 2:778318/87‑1 (MQ=255) tttgggACGTCCCTTTTAAATCCCCCCGCGGCCGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCgg < 2:517112/86‑1 (MQ=255) cTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGaa > 1:566204/1‑90 (MQ=255) tCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAggggg > 1:866528/1‑90 (MQ=255) cttttAAATCCCCCCGCGGAGGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGc < 1:93327/88‑1 (MQ=255) | TGACTGCTGGTGTTTATTTAGGGGCGTTTCCACGACATATTCCGGCACAGAATGCGGTTCTGGACGTCACCTTTGAATTCCCTCGCGGACGAGCCACAAAAGATCGACTCTATTTTTGTGTACCGATGCTGGATCTGGTGGTTCCGGAAGAGGGGGAGC > NZ_CP009273/1476070‑1476228 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |