| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,663,765 | 0 | T | A | 62.5% | 3.8 / 15.9 | 16 | I29F (ATT→TTT) | ynfL | LysR family transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base A (10/0); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.25e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.53e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCA > NZ_CP009273/1663683‑1663848 | cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGGAAAATTTAgg > 1:740225/1‑90 (MQ=255)cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGGGGAAAATTTAgg > 1:302225/1‑90 (MQ=255)cTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGGGAAAAAtttaag > 1:18802/1‑86 (MQ=255) gCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGCGGAAAATTTAAGCg > 2:761663/1‑90 (MQ=255) gcagTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAAAATTTAGGCGGGGGGCGGCGCGCCCGa > 2:300098/1‑90 (MQ=255) gcagTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGGTGGGGAAAATTTAGGGGGGGGGGGGGGCGccccg > 1:34179/1‑88 (MQ=255) gTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAAAATTCAGGGGGGGAGCGGCGCGCCCGcaaa > 2:70816/1‑89 (MQ=255) cAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCgcaacagcaac < 1:719798/90‑1 (MQ=255) gcgcCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGcaacaa < 1:423823/90‑1 (MQ=255) gctgACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGAt < 1:70816/90‑1 (MQ=255) gctgACTTAGCGGCGGTTGCGAAAAATTTAGGGGGGCGGCGGCGCGCCCCAAAAGCAGCCCTTCCCCAACAACAACAAAAAAAAGCAGaa > 1:32421/1‑89 (MQ=255) gctgACTTAGCGGCGGTTGCGAAAAATTCAGGCGGGCAGCGGCGCGCCCCAAAAGCAGCTCTTCCCCAACAGCAAAAAAGTAAAGCAGaa > 2:12318/1‑89 (MQ=255) cTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGa < 1:761663/90‑1 (MQ=255) ttAGCGGCGGTTGCGAAAAATTCAGGCGGGCAGCGGCGCGCCCCAAAAGCAGCTCTTCCCCAACAACAACAAAGAAAAGCAGATGACGaa > 2:281638/1‑90 (MQ=255) cggcggTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTc < 1:550406/90‑1 (MQ=255) gCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCa < 2:34179/90‑1 (MQ=255) | CTGCGATTGGTTCGTGCCAGCAGTCGGGCACCAATTTGTTGCTCCAGCGCCTGAATCTGCTGACTTAGCGGCGGTTGCGAAATATTCAGGCGGGCAGCGGCGCGCCCGAAATGCAGCTCTTCCGCAACAGCAACAAAGTAACGCAGATGACGAAGTTCAATATTCA > NZ_CP009273/1663683‑1663848 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |