| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,679,006 | 0 | A | C | 68.8% | ‑0.1 / 11.6 | 16 | D164A (GAC→GCC) | tus | DNA replication terminus site‑binding protein |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/11); total (5/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.29e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.03e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAA‑TGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACA > NZ_CP009273/1678917‑1679081 | ccccACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAA‑TGCTTACCGCACGCTCACCGTTCTGCACGa > 2:712969/1‑90 (MQ=255) gcgGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAA‑TGCTTACCGCACGCTCACCGTTCTGCACGACCCCGcc > 1:425/1‑90 (MQ=255) tttttGAAGGGGTGCATCGTCTTTTGCCGGGGCTGATCCCCCTTAA‑TGCTTCCCGCACGCTCCCCGTTCTGCACGCCCCCGCCACTTTAc < 2:285320/89‑1 (MQ=255) ggggGCATCGTTTTTTGCGGGGACT‑AACCCCCTTAATTGCTTCCCCCACGCTCACCGTCCTCCCCGCCCCCGCCACTTTACGCTttggtt < 1:468043/90‑1 (MQ=255) gggTGCATCGTCTTTCCCGGGGGCTTCCCCCCTTTAA‑TGCTTACCGCCCGCCCCCCTTTCTGCACGCCCCCGCCACTTTACGCTttggtt < 1:818592/90‑1 (MQ=255) gggTCCATCGTCTTTTGCGGGGGCTGACCCCCTTTAA‑TCCTTCCCGCCCCCTCACCGTTCTGCCCGCCCCCGCCACTTTACGCTttggtt < 2:627582/90‑1 (MQ=255) gggTCCATCGTCTTTTGCGGGGGCTGACCCCCCTTAA‑TCCTTCCCGCCCGCTCCCCTTTCTGCACGCCCCCGCCACTTTACGCTttggtt < 2:644338/90‑1 (MQ=255) gTGCATCTTCTTTTGCGGGGGCTGATCCCCCTTAA‑TGCTTCCCGCCCGCTCCCCGTTCTGCACGCCCCCGCCACTTTACGCTttggttgg < 2:124876/90‑1 (MQ=255) gTGCATCGTCATTTGCCGGGGCTGATCACCCTTAA‑TGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTttggttgg > 2:818592/1‑90 (MQ=255) gTGCACCGTTTTTTGCGGGGGCTGACCCCCTTTAA‑TGCTTACCGCCCGCCCCCCTTTCTGCACGCCCCCGCCACTTTACGCTttggttgg < 2:529637/90‑1 (MQ=255) gCATCCTCATTTGCCGGGGCTGACCCCCTTTAA‑TGCTCCCCGCCCGCCCCCCGTTCTGCACGCCCCCGCCACTTTACGCTTTGGTTGGGc < 1:811906/90‑1 (MQ=255) ggggCTGATCACCCTTAA‑TGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCAt > 2:41609/1‑90 (MQ=255) ggCTGACCCCCTTTAA‑TCCTTCCCCCCCCCCCCCCTTTCTGCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTa < 2:465862/90‑1 (MQ=255) tttAA‑TGCTTCCCGCCCCCTCCCCGTTCTGCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACAt < 2:695282/89‑1 (MQ=255) ttACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGtgatga > 1:661716/1‑90 (MQ=255) cGCACCCCCCCCTTTCTCCCCGCCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTc < 1:234428/90‑1 (MQ=255) cACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGcaca > 2:702046/1‑90 (MQ=255) aCCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGtgatga > 1:173476/1‑64 (MQ=255) | CCCCACCGCGGCACGTTTTGAATGGGTGCATCGTCATTTGCCGGGGCTGATCACCCTTAA‑TGCTTACCGCACGCTCACCGTTCTGCACGACCCCGCCACTTTACGCTTTGGTTGGGCTAATAAACATATCATTAAGAATTTACATCGTGATGAAGTCCTGGCACA > NZ_CP009273/1678917‑1679081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |