| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,023,591 | 0 | T | G | 58.3% | 5.9 / 12.8 | 12 | V191G (GTG→GGG) | yedA | drug/metabolite exporter YedA |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (7/0); total (8/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.01e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGCTCAGTTTATGGCTCGCGCATTACCTTACCTGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGCTTCCTTGCGGTCGGCTATCTGGCGCT > NZ_CP009273/2023511‑2023678 | tGGCTCAGTTTATGGCTCGCGCATTACCTTACCTGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGAt < 2:408304/90‑1 (MQ=255) tAGGGATGATGGCGGGTGCGGTTGAGATGCTGGCGGCAGGCGGGGGGGTAATGATCGCGGCGGTGATTGTGGGGGGAAAAATGGCggggg > 1:663460/1‑87 (MQ=255) tAGGGATGATGGCGGGGGCGATTGAGATGCTGGCGGCAGGCGGGGGGTTAATGATCGCGTCGGTGATTGGGGGGGGAAAACTGACGgcgc > 1:678838/1‑90 (MQ=255) gatgatGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGGGTTAATGATCGCGTCGATGATTGCGGGGGAAAAACTGACGGCGCtccc > 2:566749/1‑90 (MQ=255) atgatgGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGGGTTAATGATCGCGTCGATGATTGGGGGGGGAAAAATGACGGCGCcccct > 1:194492/1‑90 (MQ=255) tgatgGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGGGGTAATGATCGCGGCGATGATTGGGGGGGAAAAACTGACGGCGCtccctt > 1:577028/1‑90 (MQ=255) gatgGCGGGTGCGGTTGTGAGGCTGGCGGGCGGGGTGGGGTTAATTATCGGGGCGATGATTGGGGGGGGAAAAATGACGGGGCtcccttc > 1:796801/1‑90 (MQ=255) gatgGCGGGTGCGATTGAGATGCTGGCGGCAGGAGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCtcccttc < 2:482263/90‑1 (MQ=255) ggTGCGATTGAGATGCTGGCGGCAGGCGGGGGGTTAATGATCGCGTCGATGATTGCGGGGGAAAAACTGACGGCGCTCCCTTCCCTTTCa > 2:803567/1‑90 (MQ=255) cTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGCTTCCTTGCGGTc < 1:628782/90‑1 (MQ=255) gcggcAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGCTTCCTTGCGGTCGGc < 2:847408/90‑1 (MQ=255) ggCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGCTTCCTTGCGGTCGGCTATCTg > 2:135952/1‑90 (MQ=255) tggtgTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGCTTCCTTGCGGTCGGCTATCTGgcgc < 1:663560/90‑1 (MQ=255) ggtgTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTTAGGCTTCCTTGCGGTCGGCTATCTGGCGCt > 1:829936/1‑90 (MQ=255) ggtgTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGCTTCCTTGCGGTCGGCTATCTGGCGCt < 1:661933/90‑1 (MQ=255) | TGGCTCAGTTTATGGCTCGCGCATTACCTTACCTGTAGGGATGATGGCGGGTGCGATTGAGATGCTGGCGGCAGGCGTGGTGTTAATGATCGCGTCGATGATTGCGGGTGAAAAACTGACGGCGCTCCCTTCCCTTTCAGGCTTCCTTGCGGTCGGCTATCTGGCGCT > NZ_CP009273/2023511‑2023678 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |