Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092734,086,4990CA56.0% 7.6 / 33.6 25V100V (GTG→GTT) rhaML‑rhamnose mutarotase
Reads supporting (aligned to +/- strand):  ref base C (3/8);  new base A (14/0);  total (17/8)
Fisher's exact test for biased strand distribution p-value = 1.53e-04
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.48e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

AGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGC  >  NZ_CP009273/4086410‑4086581
                                                                                         |                                                                                  
agaTGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAac                                                                                    <  2:366113/90‑1 (MQ=255)
           gTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAg                                                                         >  2:463435/1‑90 (MQ=255)
            tttAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGGAAAAACCTTTTTCAGc                                                                        >  2:444358/1‑90 (MQ=255)
            tttAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGc                                                                        >  2:265862/1‑90 (MQ=255)
            tttAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTGCAGc                                                                        >  1:161714/1‑90 (MQ=255)
            tttAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTGCAGc                                                                        >  2:274485/1‑90 (MQ=255)
              tAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGc                                                                           >  2:137950/1‑85 (MQ=255)
              tAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGc                                                                           <  1:137950/85‑1 (MQ=255)
                aTTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGc                                                                    <  1:299350/90‑1 (MQ=255)
                                   aaaaTATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATc                                                 <  2:538861/90‑1 (MQ=255)
                                    aaaTATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATcc                                                <  1:335934/90‑1 (MQ=255)
                                          tgtgCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCCCCACTCACCCGGCTGTTTTTCGGGGTa                                          >  1:363633/1‑90 (MQ=255)
                                           gtgCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAg                                         <  2:800646/90‑1 (MQ=255)
                                           gtgCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCGCTACTCACCCGGCTGTTTTTCGGGGTAg                                         >  1:381334/1‑90 (MQ=255)
                                             gCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCg                                       <  2:161714/90‑1 (MQ=255)
                                                       tctcTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGGATAACAt                             >  2:47202/1‑90 (MQ=255)
                                                        ctctGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATc                            <  1:579487/90‑1 (MQ=255)
                                                             gCACATTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCGCTACTCACCCGGCTGTTATCCGGGGTAGCGGGCATAAAATCGGTCa                       >  2:449386/1‑90 (MQ=255)
                                                               acaTTGGGCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCCCTACTCACCCGGCTGTTTTCCGGGGTAGCGGGCATAAAATCCGGCata                     >  1:366778/1‑90 (MQ=255)
                                                                 aTTGGGCAATTACGGCAGGTAAAAAACTTCTTTCAGCTCGCCACTCCCCCGGCTGTTATCCCGGGTAGGGGGCATAAAATCGGGCatata                   >  1:165414/1‑90 (MQ=255)
                                                                 aTTGGGCAATTACGGCAGGTAAAAAACTTCTTGCAGCTCGCTACTCACCCGGCCGGTATCCGGGTTAGCGGGCAAAAAATCGGTCatata                   >  2:579487/1‑90 (MQ=255)
                                                                  ttGGGCAATTACGGCAGGTAAAAAACTTCTTGCAGCTCGCTACTCACCCGGCTGTTATCCGGGGTAGCGGGCATAAAATCGGTCatatat                  >  2:173159/1‑90 (MQ=255)
                                                                    gggCAATTACGGCAGGGAAAACACTTCTTTCAGCTCGCTACTCACCCGGCTGTTTTCCGGGTTAGCGGGGAAAAACTCGGGCATATAttt                >  1:156916/1‑90 (MQ=255)
                                                                    gggCAATTACGGCAGGGAAAAAACTTCTTTCAGCTCGCTACTCACCCGGCTGTTTTTCCGGGTAGGGGGCATAACATCGGTCCTATAttt                >  1:734338/1‑90 (MQ=255)
                                                                    gggCAATTACGGCAGGGAAAAAACTTCTTGCAGCTCGCTACTCACCCGGCTGTTATCCGGGGTAGCGGGCATAAAATCGGGCATATAttt                >  2:596048/1‑90 (MQ=255)
                                                                         aTTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTccacc           <  2:700824/90‑1 (MQ=255)
                                                                                  gggaaaaaaCTTCTTTCAGCTCGCTACTCCCCCGGCTGTTTTCCGGGGTAGCGGGCATAACATCCGGCATATATTTTCCCCCACGTTGGc  >  1:769780/4‑90 (MQ=255)
                                                                                         |                                                                                  
AGATGTAATATGTTTAATTTTTCAAATCGAAATTTAAAATATTGTGCCGGAGGCATCTCTGGCACATTGGGCAATTACGGCAGGTAAAACACTTCTTGCAGCTCGCTACTCACCGGGCTGTTATCCGGGTTAGCGGGCATAACATCGGTCATATATTTCCACCAACGTTGGC  >  NZ_CP009273/4086410‑4086581

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑