Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,791	G→A	intergenic (+160/‑32)	kefC → / → folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,791	0	G	A	100.0%	87.8 / NA	25	intergenic (+160/‑32)	kefC/folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (17/8);  total (17/8)

GAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGG  >  NZ_CP009273/49706‑49871                                                                                      |                                                                                 gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGc                                                                              >  1:176881/1‑90 (MQ=255) gagagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGc                                                                              >  2:742239/1‑90 (MQ=255) gagagCGTGGACTCGACAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGc                                                                              >  1:199838/1‑90 (MQ=255)  agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCg                                                                             >  1:19605/1‑90 (MQ=255)  agagCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCg                                                                             >  1:651594/1‑90 (MQ=255)     gCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACa                                                                          >  1:616413/1‑90 (MQ=255)       gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAt                                                                        >  1:470966/1‑90 (MQ=255)       gTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAt                                                                        >  2:362775/1‑90 (MQ=255)        tGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAtt                                                                       >  2:676281/1‑90 (MQ=255)             tCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAttttttt                                                                  >  2:454285/1‑90 (MQ=255)              cGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAAtttttttt                                                                 >  1:307310/1‑90 (MQ=255)                ccAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTAt                                                               <  2:63705/90‑1 (MQ=255)                        atatAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAt                                                       <  2:435413/90‑1 (MQ=255)                          ataAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAAtct                                                     <  1:136488/90‑1 (MQ=255)                                  ttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc                                             >  2:714036/1‑90 (MQ=255)                                  ttCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATc                                             <  2:288838/90‑1 (MQ=255)                                               tcCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcgg                                <  1:454285/90‑1 (MQ=255)                                                 cTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTgcggcg                              <  2:822486/90‑1 (MQ=255)                                                          gTCGACGACGGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTa                     >  1:729309/1‑90 (MQ=255)                                                                 acgGTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGCAGATcgcg              >  2:749236/1‑90 (MQ=255)                                                                    gTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaa                                                         >  2:280506/1‑44 (MQ=255)                                                                    gTTTACGCTTTACGTATAATGGCGACAATTTTTTTTATCGGGaa                                                         <  1:280506/44‑1 (MQ=255)                                                                             ttACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt                            <  2:244209/64‑1 (MQ=255)                                                                             ttACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGtt                            >  1:244209/1‑64 (MQ=255)                                                                             ttACGTATAATGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATgg  >  2:778744/1‑90 (MQ=255)                                                                                      |                                                                                 GAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAAATCTCAATGATCAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGG  >  NZ_CP009273/49706‑49871

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑