| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 595,037 | 0 | A | G | 62.5% | ‑2.3 / 13.0 | 16 | I290V (ATC→GTC) | cusA | Cu(+)/Ag(+) efflux RND transporter permease subunit CusA |
| Reads supporting (aligned to +/- strand): ref base A (1/5); new base G (9/1); total (10/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.62e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGC > NZ_CP009273/594961‑595123 | ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGGTCCgg > 1:226599/1‑90 (MQ=255)ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCTGCGATCCgg > 1:227967/1‑90 (MQ=255)ggTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCgg > 2:482911/1‑90 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGGTCTGCGAGCCGGCCAAAAAGcc > 1:758406/1‑90 (MQ=255) ggCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGGTCCGGCAAAAAAGcc > 2:109821/1‑90 (MQ=255) ggAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCgaga < 2:724717/90‑1 (MQ=255) ggggCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGGGGGGGGGGGGTCCCGCGATCCGGCAAAAAAGCCCGAGAAGGGGGcgccg > 1:13885/1‑90 (MQ=255) aTTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCTGCGATCCGGCCAAAAAGCCCGGGAAGGGGTCGccgccggg > 1:124632/1‑88 (MQ=255) ttGCCGAACTAAACGGCGAAGGCGAAGTGGTGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGa > 1:616139/1‑90 (MQ=255) aaaCGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTTCTGCGGTTCGGCAAAAAAGCCCCCGAAGGGGTCGCCGCCGCGGAAGACaaaat > 2:665080/1‑88 (MQ=255) aaaCGGCGAAGGCGAAGTGGCGGGCGGGGGGGGGGTCCCGCGATCCCGCAAAAAAGCCCCCGAAGGGATCGCCGCCCGGAAGGACAAACt > 1:116656/1‑90 (MQ=255) gcgaaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGaaa < 1:109821/90‑1 (MQ=255) aaggcgaagTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGc < 2:15505/90‑1 (MQ=255) cgggcgggGTGGTGGTCTTGCGATCCGGCAAAAACGCCCGAAAAGTGAACGCCGCCGTGAAGGACAAACTGGAAGCGCTGAAAAGTAGTc < 1:458937/90‑1 (MQ=255) ggtggtGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGa < 2:758406/90‑1 (MQ=255) gtgATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGc < 2:116656/90‑1 (MQ=255) | GGTCCAGATTGGCCCGGAGATGCGCCGGGGCATTGCCGAACTAAACGGCGAAGGCGAAGTGGCGGGCGGGGTGGTGATCCTGCGATCCGGCAAAAACGCCCGAGAAGTGATCGCCGCCGTGAAGGACAAACTGGAAACGCTGAAAAGTAGTCTGCCGGAAGGC > NZ_CP009273/594961‑595123 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |