| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 700,996 | 0 | C | A | 60.0% | ‑2.4 / 10.4 | 15 | P533T (CCG→ACG) | nagE | PTS N‑acetyl glucosamine transporter subunit IIABC |
| Reads supporting (aligned to +/- strand): ref base C (3/3); new base A (9/0); total (12/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.40e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.65e-01 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGA > NZ_CP009273/700911‑701081 | gATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGAc < 1:364264/90‑1 (MQ=255) ttACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGAAAACCACAg > 1:678310/1‑90 (MQ=255) tGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGGGGAAACCACAGaaaaaaaagt > 1:208681/1‑86 (MQ=255) tGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGGAAACGACAGATAAAAAAGTCGGATCACCAg > 1:359309/1‑90 (MQ=255) ccTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAAACGTCGTATCACCAGCCGCAGGGGCa > 2:237453/1‑90 (MQ=255) ccTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAAACGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACa > 2:168918/1‑90 (MQ=255) ccTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAAACGACAGATAAAAACGTCGTATCACCAGCCGCAGGGACa > 2:258642/1‑90 (MQ=255) gCAAAGCGGTGGGTGACGGTGTGGCGGTGGAAACCAAAAAAAAAAAAGTCGTATCACCAGCCCCCGGGGGAAACGGGAAAAACTTTaaaa > 1:710762/1‑88 (MQ=255) gtgggtgACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCAc < 2:92481/90‑1 (MQ=255) aCGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCt < 1:362210/90‑1 (MQ=255) aCGGTGTGGCGGTGAAAACGACAGATAAAAACGGCGTATCACCCGCCGCAGGGGCAAACGTGAAAAACTTCAACACCAACCACGCGTTCt > 2:317732/1‑90 (MQ=255) gtgtGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGGCAAACGTGAAAAACTTCAACACCAACCACGCGTTCTGcc > 2:759326/1‑90 (MQ=255) gtgtGGCGGTGAAAACCACAGATAAAAACGTCGTATCACCAGCCGCAGGGGCAAACGGGAAAAACTTCAACACCCACCACCCGGTCTGcc > 1:312911/1‑90 (MQ=255) gAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGGCAATCGTGAAAAACTTCAAAACCAACCACCCGTTCTGCCTGGAAACCGa > 1:534799/1‑90 (MQ=255) gAAAACGACAGATAAAAACGTCGTATCACCAGCCGCAGGGGCAATCGTGAAAAACTTCAACACCAACCACGCGTTCTGCCTGGGAACCGa > 1:574205/1‑90 (MQ=255) | GATTACCGGTGATGTCGTGGCACTGGATCAGGTTCCTGACGAAGCATTCGCCAGCAAAGCGGTGGGTGACGGTGTGGCGGTGAAACCGACAGATAAAATCGTCGTATCACCAGCCGCAGGGACAATCGTGAAAATCTTCAACACCAACCACGCGTTCTGCCTGGAAACCGA > NZ_CP009273/700911‑701081 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |