| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 799,325 | 0 | G | T | 54.5% | 1.6 / 11.5 | 11 | V123L (GTG→TTG) | ybhJ | hydratase |
| Reads supporting (aligned to +/- strand): ref base G (1/4); new base T (6/0); total (7/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.05e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA > NZ_CP009273/799248‑799412 | gcgcCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCCCCCCata > 2:503056/1‑90 (MQ=255) aTTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCCCCCCCTATTTCGGGCCTCCCTCaa > 2:84142/1‑90 (MQ=255) cGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCCCATATTTCGGGCATCCATCAatatat > 2:327204/1‑90 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTTTGCCTCCCCCTATTTCGGGCATCCATCCATATATTCg > 2:767875/1‑90 (MQ=255) tgatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCCCCTATTTCGGTCATCCCCCAAAATATGCg > 2:683712/1‑90 (MQ=255) gatgaCCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGt < 1:610105/90‑1 (MQ=255) tATCGGCGGCCCAGCGTTATGGCGGGATTTTTTTGCCTCCCCCTATTTGGGGCATCCCTCAATATATGCGTGGGATGATGGGAggggggg > 2:542467/1‑88 (MQ=255) aTGGCGGTATTTTTGTGCCTCCGCATATTGCGGTc > 1:129949/1‑35 (MQ=255) aTGGCGGTATTTTTGTGCCTCCGCATATTGCGGTc < 2:129949/35‑1 (MQ=255) ggTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGAc < 1:542467/90‑1 (MQ=255) ttGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCcaca < 1:753195/90‑1 (MQ=255) | GCGCCGTCGGCGGCACTATTAACGGTGATGACCATGTTTTTGGTTTATCGGCGGCCCAGCGTTATGGCGGTATTTTTGTGCCTCCGCATATTGCGGTCATCCATCAATATATGCGTGAGATGATGGCAGGCGGCGGCAAAATGATCCTCGGGTCAGACAGCCACA > NZ_CP009273/799248‑799412 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |