| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 937,169 | 0 | T | G | 52.6% | 9.0 / 24.6 | 19 | V252G (GTG→GGG) | dmsA | dimethylsulfoxide reductase subunit A |
| Reads supporting (aligned to +/- strand): ref base T (0/9); new base G (10/0); total (10/9) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.24e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCG > NZ_CP009273/937087‑937252 | aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAc < 1:220798/90‑1 (MQ=255) gCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTACt > 2:706842/1‑90 (MQ=255) gAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTTCTATCTTTAACAGGCa > 1:69181/1‑90 (MQ=255) tAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGaa < 2:193583/90‑1 (MQ=255) gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAATCTAAt > 2:45894/1‑90 (MQ=255) ggTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatc < 1:444705/90‑1 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGCATGatcatcatc > 2:159480/1‑90 (MQ=255) aaCCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTAATATCTTTAACAAGCACGCCAGAAAACTAATTGCCGCACGatcatcatc > 1:551302/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGGTTACTATTTTGAACAGGCACGCCCGAAAAAGAAAGGCCGCATGATCATCATCGATcc > 1:651388/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGGACGCCCGAAAAATAAAGCCCCCCTGATCATCATCGATcc > 1:335868/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTACTATCTTTAAAAAGGACGCCCGAAAAATAATTGCCGCCTGGACATCATCAATcc > 1:749046/1‑90 (MQ=255) gAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCgcgc < 2:280798/90‑1 (MQ=255) aaaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCt < 2:843364/90‑1 (MQ=255) aaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCta < 1:706842/90‑1 (MQ=255) aCGCGAATGAGTGGCGGTGGGGGGACTTACTATCTTGAACAGGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCCCGCtat > 2:643009/1‑90 (MQ=255) gAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCg < 2:426302/90‑1 (MQ=255) gAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCg < 1:479060/90‑1 (MQ=255) gAATGAGTGGCGGTGGGGGGGCTTACTATCTTGAACAGGCACGCCAGAAAACTAAAGCCCGCCTGATCATCATCGATCCGCGCCATACCg > 1:200221/1‑90 (MQ=255) gTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCg < 2:205675/90‑1 (MQ=255) | AGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCCG > NZ_CP009273/937087‑937252 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |