| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,544,920 | 0 | A | C | 52.9% | 11.9 / 16.9 | 17 | N68T (AAC→ACC) | fdnH | formate dehydrogenase N subunit beta |
| Reads supporting (aligned to +/- strand): ref base A (5/3); new base C (0/9); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.66e-02 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTG > NZ_CP009273/1544840‑1544999 | cTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGa > 1:146530/1‑90 (MQ=255) gTCGGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCa < 2:295915/90‑1 (MQ=255) gTCGGGGGGGGTTTTCGGAGTGGAACGACACCCGTGAGGAAGGGGGGCCCTGCGGGGGGTTTTACGATACCCCCGCCGATCTGAGCGCCa < 1:571105/90‑1 (MQ=255) gTCGGGGGGGGTGTTGGGGGTGGAACGACATCCGTGATGAAGGGGGGCACTGCGTGGGGTTTTACGATAACCCCGCCGATCTGAGCGCCa < 2:790438/90‑1 (MQ=255) gTCGGGGGGCGTGTTGGGGGGGGAACGACATCCGTGATGAAGTGGGGCCCTGCGTGGGGTTTTACAATACCCCCGCCGATCTGAGCGCCa < 1:142800/90‑1 (MQ=255) gTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGTTTTACGATAACCCCGCCGATCTGAGCGCCa < 2:276583/90‑1 (MQ=255) gTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATACCCCCGCCGATCTGAGCGCCa < 2:108055/90‑1 (MQ=255) gtgtTCGGAGTGGAACGACACCCGTGATGAAGGGGGGCACTGCGTCGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGAc < 2:253153/90‑1 (MQ=255) gAGTGGACCGACATCCGTGATGAGGGGGGGCACTGCGTGGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATg < 2:255343/90‑1 (MQ=255) gACATCCGTGATGAAGTGGGGCACTGCGTGGGGGTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGc < 2:386265/90‑1 (MQ=255) aCATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCg > 1:378045/1‑90 (MQ=255) ccGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAAc > 1:696806/1‑90 (MQ=255) cGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAAcc > 1:5558/1‑90 (MQ=255) atgaGGGGGGGCACTGCGGGGGGTTTTACGAAACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAAc < 1:659769/90‑1 (MQ=255) gCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTgg > 1:487512/1‑90 (MQ=255) ttttACAATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTg < 2:816351/89‑1 (MQ=255) gTTTACGATACCCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTg < 2:146530/90‑1 (MQ=255) | CTGTAAAGCCTGTCAGGTGGCGTGTTCGGAGTGGAACGACATCCGTGATGAAGTGGGGCACTGCGTCGGGGTTTACGATAACCCCGCCGATCTGAGCGCCAAGTCCTGGACGGTGATGCGCTTTAGCGAAACCGAACAGAACGGCAAGCTGGAGTGGCTG > NZ_CP009273/1544840‑1544999 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |