| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,759,042 | 0 | T | A | 47.8% | 20.8 / 11.9 | 23 | intergenic (‑399/+149) | sufA/ydiH | Fe‑S cluster assembly scaffold SufA/YdiH family protein |
| Reads supporting (aligned to +/- strand): ref base T (12/0); new base A (0/11); total (12/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.40e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATA > NZ_CP009273/1758967‑1759111 | tttCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgcc > 2:273614/1‑90 (MQ=255)tttCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgcc > 2:224720/1‑90 (MQ=255)tttCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACccgcc > 1:754918/1‑90 (MQ=255) ccATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAac > 1:819436/1‑90 (MQ=255) cacccGGCTTTTATCCCCGCCCCTCTCAGGGGTTTAGCTTTGGAGGGGGAAAAAAAAAAAATCCCAAAAAGCTACCCCGCCGTTTTAaca < 1:78697/87‑1 (MQ=255) cGGCGTTTATCCCCGCCACTCTCGGTGGTTTAGCTTTTGAGGGGGAAAAAAAAAAAATCTAAAAAAGCTACCCCGCCGTTTTAACACaaa < 1:372511/90‑1 (MQ=255) ggCGTTTATCTCCGCCACTCCCAGTGGTTTAGCTCTTGAAGGGGCAAAAAAAAAAATCTCAAAAACCAACCCCGCCGTTTTAACACAAAc < 2:819436/90‑1 (MQ=255) aTCTCCGCCCCTCTCAGTGGTTTAGCTTTTGAGGGGGCAAAAAAAATAATCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGAtt < 1:224720/90‑1 (MQ=255) gCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGtatta > 2:454460/1‑90 (MQ=255) tctcAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTAtttt > 2:205221/1‑90 (MQ=255) gTGGTTTAGCTTTAGAGGGGGAAAAAAAAATAATCACAAAAAGCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAAc < 1:291064/90‑1 (MQ=255) gTGGCTTGCCTTTTGAAGGGGAAAAAAAAAAAATCTAAAAAACCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAAc < 1:22766/90‑1 (MQ=255) tAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATc > 1:708554/1‑90 (MQ=255) tAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATc > 2:312875/1‑90 (MQ=255) aggCTCTGGAGGGGGCAAAAAAAAAAATCTTAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATc < 2:811433/88‑1 (MQ=255) tttGAGGGGGCAAAAAAAATAATCTCAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCg < 2:131272/89‑1 (MQ=255) tttGAAGGGGAAAAAAAAATAATCAAAAAAAGCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCg < 1:409382/89‑1 (MQ=255) ttagAAGGGGAAAAAAAAATAATCTAAAAAAGCAACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCg < 1:601757/87‑1 (MQ=255) ctTGAGGGGGCAAAAAAAATAATCTAAAAAAGCTACCCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCg < 2:80512/90‑1 (MQ=255) ttGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCgg > 1:718706/1‑90 (MQ=255) ttGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCgg > 2:739229/1‑90 (MQ=255) gggCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGact > 1:811433/1‑88 (MQ=255) gggCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATa > 2:376640/1‑90 (MQ=255) | TTTCTACCCCATCCGGCGCTTATCTCCGGCACTCTCAGTGGCTTAGCTCTTGAAGGGGCGATAAGAATAATCTCATAAAGCTAACCCGCCGTTTTAACACAAACTGCGATTAGTATTATTTTTGAACAATATCAGGCGGTAGATA > NZ_CP009273/1758967‑1759111 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |