| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,214,126 | 0 | T | C | 52.6% | 17.2 / 27.7 | 19 | I425T (ATC→ACC) | ygjG | putrescine aminotransferase |
| Reads supporting (aligned to +/- strand): ref base T (9/0); new base C (0/10); total (9/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.08e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 7.68e-05 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTA > NZ_CP009273/3214048‑3214214 | aaaTCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAAc > 2:53064/1‑90 (MQ=255) tttGCAAGGGAGTTGTCCCGCCGGCGCGTACTGGGGGCCGGACCGCTAAAAAACCCAAAAACGACCCGCATTGAACCGCcactgacactg < 2:645161/90‑1 (MQ=255) tttCCCAGCGAGTTGTCCCGCCAGCGCGTCCGGGGGGCCGGACCGCTAAAAACCCCAAAAACGACCCGCATTGAACCGCcactgacactg < 2:719759/90‑1 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 2:764143/1‑90 (MQ=255) ttGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCcactgacactga > 1:573086/1‑90 (MQ=255) tGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCactgacactgac > 1:701056/1‑90 (MQ=255) gagaTGTCCCGCGGGGGGGTCCGGGGGGCGGAAACGCCAAAAAACGAAAAAACGCCCCGCATTAACCCGCCACTGACACTGACCATTGaa < 1:612284/90‑1 (MQ=255) agaTGTCCCCCCGGCGCGTACTGGGGGCCGGAACGCTAAAAACCGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAAc < 2:255735/90‑1 (MQ=255) ccGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGa > 2:265120/1‑90 (MQ=255) gCCAGCGCGTACTGGGGGCCGGAACGCTAAAAAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAAc < 2:243022/90‑1 (MQ=255) ggcgcGTCCGGGGGGCCGAAACCCTAAAAAACCCAAAACCGACCCGAATTAAACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg < 1:50823/89‑1 (MQ=255) aGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTgg > 1:271124/1‑90 (MQ=255) ggggCGGGACCGCTAAATAACGCAAAAACGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcg < 2:49490/88‑1 (MQ=255) ggCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAgcggc > 2:534635/1‑90 (MQ=255) aCGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTggcgg > 1:562399/1‑90 (MQ=255) caaaaaCGACCCGAATTGACCCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCa < 2:125871/88‑1 (MQ=255) caaaaaCGACCCGAATTAACCCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCa < 2:746037/88‑1 (MQ=255) aaaCGACCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGc < 2:93136/90‑1 (MQ=255) aTCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTa > 1:815547/1‑90 (MQ=255) | AAATCGGCTATAACTTTGCCAGCGAGATGTTCCGCCAGCGCGTACTGGTGGCCGGAACGCTCAATAACGCCAAAACGATCCGCATTGAACCGCCACTGACACTGACCATTGAACAGTGTGAACTGGTGATCAAAGCGGCGCGTAAGGCGCTGGCGGCCATGCGAGTA > NZ_CP009273/3214048‑3214214 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |