| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,485,387 | 0 | C | T | 53.1% | 7.2 / 43.0 | 33 | Q90Q (CAG→CAA) | ppiA | peptidylprolyl isomerase A |
| Reads supporting (aligned to +/- strand): ref base C (0/15); new base T (17/0); total (18/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.77e-09 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.33e-06 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGC > NZ_CP009273/3485301‑3485472 | tCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTctgc < 1:35799/90‑1 (MQ=255) cAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTctgct < 1:336380/90‑1 (MQ=255) gCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTTGTTTTTTTttctgc > 2:610871/1‑90 (MQ=255) gCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTTGTTTTTTTttctgc > 2:336380/1‑90 (MQ=255) gCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGGGTTTTTTttctgc > 2:358202/1‑90 (MQ=255) cATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTTGTTTTTTTTGCTGCCTCTGCTCggg > 2:143831/1‑89 (MQ=255) cATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGGGTTTTTTTTCTGCCTCTTCTCggg > 2:740618/1‑89 (MQ=255) tCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGGTTTTTTTTGTTGCCTCTTCTCGGT‑gg > 1:34904/1‑89 (MQ=255) ccACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGccc < 1:143831/90‑1 (MQ=255) aCGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTTGTTTTTTTTGCTGCATCTTCTCGGG‑GAAAACGcccccccg > 2:381148/1‑88 (MQ=255) aCGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGGTTTTTTTTTCTGCTTCTGCTCGGTGGAAACccccccccc > 1:778305/1‑89 (MQ=255) aCGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGGTTGGTTTTTTTTGCTGCATCTGCTCGGG‑GAAAACGCCGCCCTg > 2:260605/1‑90 (MQ=255) gcgTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTTTTTTGTATCTTCTCTTT‑GGAAACGcccccccgga > 1:599189/1‑86 (MQ=255) gcgTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGaa < 2:545749/90‑1 (MQ=255) gcAGGCCGTTATCGGCTTCATTTTTGATTGGCGGGGTTTGGTTTTTT‑TGCTGGCCCTGGTCGGG‑GTAAACGCCCCCCCCAATAATaaaac > 1:46708/1‑88 (MQ=255) gcAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTTTGTTGCATTTGCTCGGG‑GAAACCGCCCCCCTGAAACATaaaac > 2:90183/1‑88 (MQ=255) cGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgc < 1:88280/90‑1 (MQ=255) cGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgc < 2:232461/90‑1 (MQ=255) cGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgc < 2:321704/90‑1 (MQ=255) cGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgc < 1:430507/90‑1 (MQ=255) cGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgc < 1:272567/90‑1 (MQ=255) cGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgc < 1:358202/90‑1 (MQ=255) cGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGAcgc < 2:450305/90‑1 (MQ=255) ggCTTCATTTTTGATTGGC‑GGGTTTTGTTTTTTTTTCTGCATCTTCTCGGG‑GAAAACGCCGCCCCGGATCATAAAACCAGGAAAGAcgcg > 2:699014/1‑90 (MQ=255) ggCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTTTGCTGCATCTGCTCGGG‑GAAACCGCCGCCCTGAATCATAAAGCCAGGGATGAcgcg > 2:25775/1‑90 (MQ=39) ggCTTCATTTTTGATTGGC‑GGGGTTGGGTTTTTTTTCTGCATCTTCTCGGT‑GGAAACCCCCCCCTGAATCATAAAAACAGGAATGAggcg > 2:88280/1‑90 (MQ=255) gCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCgg < 1:806361/90‑1 (MQ=255) gCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCgg < 1:27219/90‑1 (MQ=255) cTTCATTTTTGATTGGC‑GGGGTTGGGTTTTTTTTCTGCCTCTTCTCGGT‑GGAAACGCCCCCCCGAAACATAAAACCCGGAAAGACGCggg > 1:292890/1‑89 (MQ=255) cTTCATTTTTGATTGGC‑GGGGTTGGGTTTTTTTTCTGCCTCTGCTCGGT‑GGAAACCCCCCCCCGAATCATAAAACCAGGAATGACGCggg > 1:59129/1‑89 (MQ=255) ttCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTg < 2:448772/90‑1 (MQ=255) tCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGa < 1:381148/90‑1 (MQ=255) tCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGa < 1:409726/90‑1 (MQ=255) tCATTTTTGATTGGC‑GGGGTTGGTTTTTTTTTCTGCATCTGCTCGGG‑GAAAACGCCGCCCTGAATCATAAAAACAGGGATGACGCggggg > 2:409726/1‑87 (MQ=255) ttttCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGc < 2:599189/90‑1 (MQ=255) | TCAGCGGTACGTGCCATCGCGATGGTGCCACGCGTGTTGCGCAGGCCGTTATCGGCTTCATTTTTGATTGGC‑GGGTTTGGTTTTTTCTGCTGCATCTGCTCGGT‑GAAACCGCCGCCCTGAATCATAAAGCCAGGAATGACGCGGTGAAAGGTAGTGTTGTTATAAAAACCGC > NZ_CP009273/3485301‑3485472 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |