| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 71,567 | 0 | A | T | 57.1% | 2.1 / 12.3 | 14 | L134Q (CTG→CAG) | thiB | thiamine ABC transporter substrate binding subunit |
| Reads supporting (aligned to +/- strand): ref base A (1/5); new base T (8/0); total (9/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.00e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
ACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGA > NZ_CP009273/71485‑71644 | aCTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGgttttttgtttttt > 1:259540/1‑90 (MQ=255) tGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTtgtttgtttt > 2:488330/1‑88 (MQ=255) ggTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTtttttttt > 1:413057/1‑90 (MQ=255) ggTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTtttttttt > 2:32186/1‑90 (MQ=255) gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACTAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTttttttttttt > 2:723483/1‑87 (MQ=255) gcgcGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTGtt > 2:560091/1‑89 (MQ=255) tCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTGTTTTTTTCATaaaaa > 2:298946/1‑88 (MQ=255) aaTCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTTTTTAACAAAGGCgga > 1:282340/1‑88 (MQ=255) tCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGt < 1:464191/90‑1 (MQ=255) ccGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGcc < 1:468219/90‑1 (MQ=255) tttGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCaa < 1:298946/90‑1 (MQ=255) gCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGGTTTTTTGGTTTTTTTTGTTCTAAAACAAGGGGGAGTAGCCATAAACAAAAGGGAc > 2:411010/1‑90 (MQ=255) gTTCTTTCAGGCTTTGTGGCGGGGTTTTTAGTTTGTTTTTTTTATAAAAGAAGGCGAAGGAGCCATAATCAAAAGGGACGAAAGAGTCat > 2:90926/1‑90 (MQ=255) tttCAGGCTTTGTGGCGGGGTTTTTTGTTTTTTTTTTTCTTAAACAAAAGGGGAGGAGCCAGAATCAAACAGGACGGAAAGGTCattatt > 1:402352/1‑90 (MQ=255) ggCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGa < 1:560091/90‑1 (MQ=255) ggCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGa < 2:259540/90‑1 (MQ=255) | ACTGGTGCGCGGATCCTGATAAATCACCCGCCAGTTTTGATCGCTCTCAACCAGTTCTTTCAGGCTTTGTGGCGGGTTTTTCAGTTTGTTCTTGTCATAAACGAAGGCGAAGTAGCCATAATCAAACGGTACGAAAGTGTCATTATTCCAGCCGCCGGGA > NZ_CP009273/71485‑71644 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |