| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,613,623 | T→G | W83G (TGG→GGG) | marR → | multiple antibiotic resistance transcriptional regulator MarR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,613,623 | 0 | T | G | 95.7% | 82.3 / ‑3.2 | 23 | W83G (TGG→GGG) | marR | multiple antibiotic resistance transcriptional regulator MarR |
| Reads supporting (aligned to +/- strand): ref base T (1/0); new base G (14/8); total (15/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.78e-01 | |||||||||||
TATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAAT > NZ_CP009273/1613538‑1613707 | tATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCggggg > 1:323865/1‑89 (MQ=255) ccGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAgg > 2:190220/1‑90 (MQ=255) ccGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGGGGAAAgg > 1:187820/1‑90 (MQ=255) ccGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGGGGAAAgg > 1:259973/1‑90 (MQ=255) ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAgggt > 2:98396/1‑88 (MQ=255) ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGtt > 2:545676/1‑90 (MQ=255) ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGtt > 1:716376/1‑90 (MQ=255) ggTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGGGGGAAGGtt > 1:250245/1‑90 (MQ=255) aCTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGGGGAAAGGTTGCCGaa > 1:212382/1‑90 (MQ=255) tcggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAgcgc < 2:716376/90‑1 (MQ=255) ggtcgACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCgcgg < 1:545676/90‑1 (MQ=255) gACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTa < 2:237795/90‑1 (MQ=255) gACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTa < 2:45229/90‑1 (MQ=255) gACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTa < 1:302748/90‑1 (MQ=255) gACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGGGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTa > 1:45229/1‑90 (MQ=255) aCTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGCGGGAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACt > 1:243788/1‑90 (MQ=255) tGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTa > 2:200792/1‑90 (MQ=255) aCCCGTATGCTGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTacc < 2:620593/90‑1 (MQ=255) tGGATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcg < 1:646389/90‑1 (MQ=255) ggATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgc > 1:68262/1‑90 (MQ=255) ggATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGgcggcgc > 2:111457/1‑90 (MQ=255) ggATCGCCTGGTCTGTAAAGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGGAAAACTTACCACCGgcggcgc > 1:738306/1‑90 (MQ=255) gTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATAtgt > 1:151735/1‑90 (MQ=255) aaGGCGGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAAt < 1:794829/90‑1 (MQ=255) | TATTACTCCGGTTGAACTGAAAAAGGTATTGTCGGTCGACCTGGGAGCACTGACCCGTATGCTGGATCGCCTGGTCTGTAAAGGCTGGGTGGAAAGGTTGCCGAACCCGAATGACAAGCGCGGCGTACTGGTAAAACTTACCACCGGCGGCGCGGCAATATGTGAACAAT > NZ_CP009273/1613538‑1613707 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |