| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 296,422 | 0 | G | C | 52.9% | 12.0 / 26.7 | 17 | P75R (CCG→CGG) | paoC | aldehyde oxidoreductase molybdenum‑binding subunit PaoC |
| Reads supporting (aligned to +/- strand): ref base G (3/5); new base C (9/0); total (12/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.05e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.10e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGG > NZ_CP009273/296335‑296511 | gTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAAAGACAACCCGTACGCCCCgc > 1:260433/1‑90 (MQ=255)gTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATGTGAGCCCCGACGCCCgcc > 1:790670/1‑88 (MQ=255) ggggCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGGAATACAACCCGGACGCCCCgcgc > 2:796524/1‑90 (MQ=255) gccgccTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCCGCGCttt > 1:175101/1‑90 (MQ=255) gCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGGAATGACAAGCCGGACCCCCCGCGCCTTTTTCtcggcgg > 2:387724/1‑89 (MQ=255) cGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAAGGACAGCCAGTAAGCCCCGCGCTTTTTTGGCGGGGTCCGGCTCGAGGGGGGTgg > 2:652934/1‑89 (MQ=255) cTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGc < 1:652934/90‑1 (MQ=255) ttGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCCGCGCTTTTTTCGCGGCGTCCCTATCAAAGGGGGGGGGGCgg > 1:185072/1‑89 (MQ=255) cGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCtt < 2:704525/90‑1 (MQ=255) gAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCCGTACGCCCCGCGCTTTTTTCGCGGGGTCCGTATCAAAGGGGGGGGGGGGGCCttt > 2:272950/1‑90 (MQ=255) gTGCCCCGGCGTTACTGGCGGGAAAGACAACCAGTACGCCCCGCGCCTTTTTTGCGGCGGCCGTATCAAGGGGGGGGAGGCGGCCctttg > 2:785704/1‑88 (MQ=255) cGGTAATGACAGCCAGTACGCCCCGCGCTTTTTGCGCGGCGGCCGTATCAAGGGGGGGGGGGGGGCCTTTGGCAAAGGCGGGAAccacga > 2:147994/1‑90 (MQ=255) aGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACCATATAGCCATa > 2:535440/1‑90 (MQ=255) gCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAg > 2:691110/1‑90 (MQ=255) ccAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCg < 1:74363/52‑1 (MQ=255) ccAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCg > 2:74363/1‑52 (MQ=255) cAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAggc < 1:204186/90‑1 (MQ=255) gTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAggcgg < 1:691110/90‑1 (MQ=255) ggCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTgggg > 2:450603/1‑90 (MQ=255) | GTGGGGCCGCCTAACAGCCTGGCGGTGTTTTTGTCGCCTTTGCCGAGTGCCCCGGCGTTACTGGCGGTAATGACAGCCAGTACGCCCGGCGCTTTTTGCGCGGCGTCCGTATCAAGGGCGGTGAGGCGTCCTTTGGCAATGGCGGAACCGACGATATAGCCATAGGCGGCGTTGGGG > NZ_CP009273/296335‑296511 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |