| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 333,780 | 0 | A | G | 57.1% | 6.7 / 13.6 | 14 | D431G (GAT→GGT) | fdrA | acyl‑CoA synthetase FdrA |
| Reads supporting (aligned to +/- strand): ref base A (2/4); new base G (8/0); total (10/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.50e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAA > NZ_CP009273/333693‑333868 | aGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGAGCGTGGTGGGtt > 1:811782/1‑90 (MQ=255) gACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGa < 1:550316/90‑1 (MQ=255) gACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGATGGGTTTTTTGCTCGGGTTTggg > 2:117835/1‑89 (MQ=255) caccaACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGGTGGGTTTTTTGTTCGGGGTTGGATCGCa > 2:746691/1‑90 (MQ=255) caACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGa < 2:665651/90‑1 (MQ=255) cATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGGGGTGGGTTTTTTGCTCGGGGTTGGATCGCATGAAGATcc > 2:616770/1‑90 (MQ=255) gATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTccac < 1:93844/90‑1 (MQ=255) tCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGGTGGGTTTTTTGCTCGGGTTTGGGGCGCATGAAGAACCGGTCGGGTccccca > 2:417790/1‑90 (MQ=255) ggCGGTGATCGTGATGGGTTTTTTGCTCGGGTTTGGGTCGCATGAAGATCCGGGCGGCTCCCCCATCGAGACGATCAAAAaagcgaaagc > 2:254560/1‑90 (MQ=255) ggCGGTGATCGTGATGGGTTTTTTGCTCGGGTTTGGATCGCATGAAGATCCGGGCGGCTCCACCATCGAGACGATCAAAAaaaagaaaga > 2:343527/1‑89 (MQ=255) ggCGGTGATCGTGATGGATTTTTTGCTCGGGTTTTGATCGCATGAAGATCCGGGCGGCTCCCCCCTCGAGACGATCAAAAaagcgaaaac > 2:231461/1‑88 (MQ=255) ggCGGTGATCGGGATGGGTTTTTTGCTCGGGTTTGGATCGCATGAAGATCCGGGCGGCTCCACCATCGAGACGATCAAAAaagcgaaaac > 2:537826/1‑88 (MQ=255) gTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATc < 1:254560/90‑1 (MQ=255) gATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGaa > 2:686536/1‑90 (MQ=255) | AGCCGCACCCGATGATTGACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAA > NZ_CP009273/333693‑333868 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |