| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 333,785 | 0 | G | T | 53.3% | 14.6 / 27.0 | 15 | V433L (GTG→TTG) | fdrA | acyl‑CoA synthetase FdrA |
| Reads supporting (aligned to +/- strand): ref base G (2/5); new base T (8/0); total (10/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.99e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.72e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGC > NZ_CP009273/333710‑333874 | gACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGa < 1:550316/90‑1 (MQ=255)gACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGATGGGTTTTTTGCTCGGGTTTggg > 2:117835/1‑89 (MQ=255) caccaACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGGTGGGTTTTTTGTTCGGGGTTGGATCGCa > 2:746691/1‑90 (MQ=255) caACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGa < 2:665651/90‑1 (MQ=255) cATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGGGGTGGGTTTTTTGCTCGGGGTTGGATCGCATGAAGATcc > 2:616770/1‑90 (MQ=255) gATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTccac < 1:93844/90‑1 (MQ=255) tCGAAGAGGCGCGCGATCCAGAAGTGGCGGGGATCGGGGTGGGTTTTTTGCTCGGGTTTGGGGCGCATGAAGAACCGGTCGGGTccccca > 2:417790/1‑90 (MQ=255) ggCGGTGATCGTGATGGGTTTTTTGCTCGGGTTTGGGTCGCATGAAGATCCGGGCGGCTCCCCCATCGAGACGATCAAAAaagcgaaagc > 2:254560/1‑90 (MQ=255) ggCGGTGATCGTGATGGGTTTTTTGCTCGGGTTTGGATCGCATGAAGATCCGGGCGGCTCCACCATCGAGACGATCAAAAaaaagaaaga > 2:343527/1‑89 (MQ=255) ggCGGTGATCGTGATGGATTTTTTGCTCGGGTTTTGATCGCATGAAGATCCGGGCGGCTCCCCCCTCGAGACGATCAAAAaagcgaaaac > 2:231461/1‑88 (MQ=255) ggCGGTGATCGGGATGGGTTTTTTGCTCGGGTTTGGATCGCATGAAGATCCGGGCGGCTCCACCATCGAGACGATCAAAAaagcgaaaac > 2:537826/1‑88 (MQ=255) gTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATc < 1:254560/90‑1 (MQ=255) gATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGaa > 2:686536/1‑90 (MQ=255) ttGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGAc > 1:761596/1‑90 (MQ=255) ttGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGAc < 1:263885/90‑1 (MQ=255) gtgCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGAcgc > 1:617743/1‑90 (MQ=255) gtgCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGAcgc < 1:363036/90‑1 (MQ=255) | GACCCCACCAACCGCATCAGTCGCTTGATCGAAGAGGCGCGCGATCCAGAAGTGGCGGTGATCGTGATGGATTTTGTGCTCGGATTTGGATCGCATGAAGATCCGGTCGGCTCCACCATCGAGACGATCAAAGAAGCGAAAGCGATCGCCGCTGCCGAAGGACGC > NZ_CP009273/333710‑333874 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |