| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,141,808 | 0 | T | G | 53.3% | 15.5 / 18.3 | 15 | G218G (GGT→GGG) | yegD | molecular chaperone |
| Reads supporting (aligned to +/- strand): ref base T (1/6); new base G (8/0); total (9/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.40e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.29e-03 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGG > NZ_CP009273/2141719‑2141884 | gTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCggt < 1:765179/90‑1 (MQ=255) tACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGGGGgacgacc > 1:248834/1‑89 (MQ=255) aGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGGGGGACggctgact > 1:54299/1‑90 (MQ=255) gTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCggtgg > 2:799011/1‑74 (MQ=255) gTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCggtgg < 1:799011/74‑1 (MQ=255) gctggATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGAt < 2:449610/90‑1 (MQ=255) gCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGGGGGGAGACTGACTGTTTATTGCTGCTGATGGGGGCGCAgggg > 1:191453/1‑90 (MQ=255) gCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGGGGGAAGAGTGACTGGTCTTTGCTTCTGGTGGGGGCGGCGTgg > 1:523341/1‑90 (MQ=255) cTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTc < 1:684587/90‑1 (MQ=255) cTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTc < 1:808806/90‑1 (MQ=255) cAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGGGGGACGACTGACTGTTTATTTTTGCTGGTGGGGGCGCCGGGGGGGTcgcgg > 1:652089/1‑89 (MQ=255) aagaaAAACGGGTGCTGGTGGTGGATATCGGCGGGGGGGAGACCGACTGTTCATTTCTGCTGGTGGGGGCGGCGGGGGGGTCGCGGCTCg > 2:796921/1‑90 (MQ=255) agaaAAACGGGTGCTGGTGGTGGATATCGGCGGTGGGACGACTGACTGTTTATTTCTGCTGGTGGGGGCGCAGGGGGGGTCGCGTCTCGa > 1:64242/1‑90 (MQ=255) ggTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAagc < 2:54299/90‑1 (MQ=255) ggTGCTGGTGGTGGATATCGGCGGGGGGGCGACCGAATGTTTTTTTCTGCTGGTGGGGGCGCGGGGGGGGTTGCGGCTCGCTCGTGAagc > 2:263919/1‑90 (MQ=255) tGCTGGTGGTGGATATCGGCGGGGGGGTGACTGACCATTCTTTGCTGGTGGTGGGGGCCCAGGGGGGGTTGCGGCCCGTTCGTGAaggca > 2:698480/1‑90 (MQ=255) gCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAagccag < 1:698480/90‑1 (MQ=255) tggATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTgg < 1:18683/90‑1 (MQ=255) | GTATTCCAGTACGAGCCGGTCGCGGCTGGGCTGGATTACGAAGCCACCTTGCAGGAAGAAAAACGGGTGCTGGTGGTGGATATCGGCGGTGGTACGACTGACTGTTCATTGCTGCTGATGGGGCCGCAGTGGCGTTCGCGTCTCGATCGTGAAGCCAGCCTGCTGG > NZ_CP009273/2141719‑2141884 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |