Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,145,0730GT63.6% ‑4.7 / 11.7 11L136F (TTG→TTT) yegJDUF2314 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base G (0/4);  new base T (7/0);  total (7/4)
Fisher's exact test for biased strand distribution p-value = 3.03e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.37e-02
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG  >  NZ_CP009273/2145014‑2145162
                                                           |                                                                                         
gACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTACCATTGTTTCTTTTTTTGTTACCTATCCAAAAAAAAAAGACGACGAg                                                             >  2:606479/1‑90 (MQ=255)
            aCGCGCGGGGATAAAATTTACGGCGGGTTTTACATTTTTTCTTTTTTTGTTACCCATCCAAAAAAAAAAAACGACGAGGCAAAAGGaaaa                                                 >  2:315165/1‑90 (MQ=255)
              gcgcgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTTTTTGTCACCTATCCAAAAAAAAAAACCGACGAGCTAAAAGCaaaaat                                               >  1:102809/1‑88 (MQ=255)
                gcgGGGATAAAATTTACGGCGGTTTTAACATTTATCCTTTTTTTGTGACCCATCCCAAAAAAAAAACCGACGAGCTAAAAGAAAAAATaa                                             >  2:211464/1‑89 (MQ=255)
                  ggggATAAAATTTACGGCGGTTGTTATATTAATTCTTTTTTTTTCTCCCATCCCAAAAAAAAAAACGACGAGATTAAAGGAAAAATAAtt                                           >  2:494500/1‑90 (MQ=255)
                  ggggATAAAATTTACGGCGGTTATACCATTTATCCTTTTTTTGTTACCTATCCAAAAAAAAAAAACGACGGGCCAAAAGGAAAAATAAtt                                           >  1:835056/1‑90 (MQ=255)
                             ttACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGc                                <  1:494500/90‑1 (MQ=255)
                             ttACGGCGGTTATAACATTTTTTCTTTTTTTGTTACCTATCCAAAAAAAAAAAACGACGAGCTAAGAGGAAAAATAATTTGCTAATCaac                                >  2:109324/1‑88 (MQ=255)
                                        ataCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTAtattta                     <  1:538629/90‑1 (MQ=255)
                                                      ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGATTAAACTGGc       <  2:102809/90‑1 (MQ=255)
                                                         ttGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGt    <  1:728357/90‑1 (MQ=255)
                                                           ggTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTg  >  1:816187/1‑90 (MQ=255)
                                                           |                                                                                         
GACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTG  >  NZ_CP009273/2145014‑2145162

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑