| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,145,092 | 0 | G | A | 53.8% | 3.0 / 15.6 | 13 | E143K (GAA→AAA) | yegJ | DUF2314 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (1/5); new base A (7/0); total (8/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.66e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.56e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGT > NZ_CP009273/2145014‑2145168 | gACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTTTACCATTGTTTCTTTTTTTGTTACCTATCCAAAAAAAAAAGACGACGAg > 2:606479/1‑90 (MQ=255) aCGCGCGGGGATAAAATTTACGGCGGGTTTTACATTTTTTCTTTTTTTGTTACCCATCCAAAAAAAAAAAACGACGAGGCAAAAGGaaaa > 2:315165/1‑90 (MQ=255) gcgcgGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTTTTTGTCACCTATCCAAAAAAAAAAACCGACGAGCTAAAAGCaaaaat > 1:102809/1‑88 (MQ=255) gcgGGGATAAAATTTACGGCGGTTTTAACATTTATCCTTTTTTTGTGACCCATCCCAAAAAAAAAACCGACGAGCTAAAAGAAAAAATaa > 2:211464/1‑89 (MQ=255) ggggATAAAATTTACGGCGGTTGTTATATTAATTCTTTTTTTTTCTCCCATCCCAAAAAAAAAAACGACGAGATTAAAGGAAAAATAAtt > 2:494500/1‑90 (MQ=255) ggggATAAAATTTACGGCGGTTATACCATTTATCCTTTTTTTGTTACCTATCCAAAAAAAAAAAACGACGGGCCAAAAGGAAAAATAAtt > 1:835056/1‑90 (MQ=255) ttACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGc < 1:494500/90‑1 (MQ=255) ttACGGCGGTTATAACATTTTTTCTTTTTTTGTTACCTATCCAAAAAAAAAAAACGACGAGCTAAGAGGAAAAATAATTTGCTAATCaac > 2:109324/1‑88 (MQ=255) ataCCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTAtattta < 1:538629/90‑1 (MQ=255) ttATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGATTAAACTGGc < 2:102809/90‑1 (MQ=255) ttGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGt < 1:728357/90‑1 (MQ=255) ggTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTg > 1:816187/1‑90 (MQ=255) cACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAt < 2:835056/90‑1 (MQ=255) agaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGtt > 2:277884/1‑46 (MQ=255) agaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGtt < 1:277884/46‑1 (MQ=255) agaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttgt < 1:769371/78‑1 (MQ=255) agaagCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAAttgt > 2:769371/1‑78 (MQ=255) | GACTGGATGTATACGCGCGGGGATAAAATTTACGGCGGTTATACCATTGATCCTTTATTGGTCACCTATCCAAAAGAAGAAGCCGACGAGCTAAGAGCAAAATTAGTTCGCTAATCAGCCGTTATATTTATTGTTTAAACTGGCGGTTGAATTGT > NZ_CP009273/2145014‑2145168 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |