| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 5,159 | 0 | G | A | 52.6% | 7.3 / 24.1 | 19 | intergenic (+139/‑75) | thrC/yaaX | threonine synthase/DUF2502 domain‑containing protein |
| Reads supporting (aligned to +/- strand): ref base G (8/1); new base A (0/10); total (8/11) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.19e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.61e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATG > NZ_CP009273/5089‑5245 | tGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTAAGAGATTAGGATTGCGGAGAATAACAACCGCCGTtctc > 1:671548/1‑90 (MQ=255) ggAAAAAAAAGACAGGAAAAAAGAAAAAACTCCAAAAAATTGCGGTAACTTAGAGTTTAGGATGGCGGAAAAAAACAACCGCCGTTCTCa < 2:480450/90‑1 (MQ=255) aaaaaaaTGAAGGGAAAAAAGAAAAAATTCTAAAAAAATGCGGTAACTTAGAGATTAGGTTGGGGGAGAAAAACAACCGCCGTTCTCATc < 2:58929/88‑1 (MQ=255) gaAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCg > 1:759674/1‑90 (MQ=255) aaaaaaTGACAGGAAAAAAGGAAAAATTCTCAAAAAATGCGGTAACTTAGAGATTAGGTTTGGGGAAAATAACAACCGCCGTTCTCATCg < 1:73253/89‑1 (MQ=255) tGAAAGGAAAAAAGAAAAAATCCTAAAAAAATGGGGTAACTTAGAAATTAGGATGGCGGAAAAAAACAACCGCCGTTCTCATCGAGTAAt < 2:671548/90‑1 (MQ=255) aCAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAAtct > 2:273388/1‑90 (MQ=255) aaGGAAAAATTCAAAAAAAGGGGGGTAATTAAGATTTTAGGTTGGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATc < 2:266071/90‑1 (MQ=255) agaAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAcc > 2:67853/1‑90 (MQ=255) gaAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGAcac > 1:675777/1‑88 (MQ=255) tctcAATAAATGCGGTAACTTAGAGATTAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAAc < 1:134399/90‑1 (MQ=255) tctcAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAAc > 1:693121/1‑90 (MQ=255) tcccAAAAAACGCGTTAACTAAGAGTTTAGGCTGGGGGAAAAAAAACACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAAc < 1:755444/87‑1 (MQ=255) ggTAACTTAGAGTTTAGGATTGGGGAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaa < 1:9889/90‑1 (MQ=255) gTAACTTAGAGATTAGGTTGGCGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATaaaa < 1:273388/90‑1 (MQ=255) gagaTTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAAc > 1:307212/1‑90 (MQ=255) agaTTAGGATGGGGAAAAAAAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAAcc < 1:527406/90‑1 (MQ=255) aGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGa > 1:734662/1‑90 (MQ=255) ggAAAAAAACACCCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATg < 1:652270/90‑1 (MQ=255) | TGGAGAAAAATGACAGGGAAAAAGGAGAAATTCTCAATAAATGCGGTAACTTAGAGATTAGGATTGCGGAGAATAACAACCGCCGTTCTCATCGAGTAATCTCCGGATATCGACCCATAACGGGCAATGATAAAAGGAGTAACCTGTGAAAAAGATG > NZ_CP009273/5089‑5245 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |