| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 6,981 | 0 | A | G | 55.0% | 5.2 / 14.9 | 20 | Y327H (TAC→CAC) | yaaJ | sodium:alanine symporter family protein |
| Reads supporting (aligned to +/- strand): ref base A (6/3); new base G (0/11); total (6/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 2.17e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.32e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAA > NZ_CP009273/6892‑7058 | aaCCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTa < 1:749230/90‑1 (MQ=255) aaGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTtgt > 1:417012/1‑90 (MQ=255) gTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCg > 2:370291/1‑90 (MQ=255) cccAGCCCCCCAGGACCCCATTAGCCCCCGCCTCCCCTTCTGGAAAAGCTAAATCCCTTCCAGCGGCATGTGGGTTGTGCCGTTACCCGc < 2:784027/88‑1 (MQ=255) cAGGACCCCATTCCCCCCCGCCTCCCCTTCTGAAAAAGCTAAATCCCTCCCGGGGGTTTGTGGGTTGTGCCGTTCCCCGCCAGTAATATc < 2:858582/90‑1 (MQ=255) cAGGACCCCATTACCCCCCGCATCCCCTTCTGGATAAGCTAAATCCCTCCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATc < 1:249722/90‑1 (MQ=255) cAGAAACCCATTAGCCCCCGCACCCCCTTCTGGAAAAGCTAAATCCCTCCCAGCGGCATGTAGGTGGTGCCGTTCCCCGCCAGTAATATc < 1:172314/90‑1 (MQ=255) aaGGACCCCTTTACCCCCCGCATCCCCTTCTGGAAAAGCTAAATCCCTTCCAGCGGCATGTGGGTTGTGCCGTTCCCCGCCAGTAATATc < 1:271104/89‑1 (MQ=255) ccATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATgg > 2:657064/1‑90 (MQ=255) aTTAGCCCCCGCATCCCTTTCTGGATAAGCTAAATCCCTCCCGGGGGCATGTGGGTTGTGCCTTTCCCCGCCAGTAATATCAGCATGgcg < 2:360727/90‑1 (MQ=255) accccccGCATCGCCTTCTGGAAAAGCAAAACCCCTCCCAGGGGGTTGGGGGTTGTGCCTTTCCCCGCCAGTAATATCAGCATGGCGCtt < 2:813224/86‑1 (MQ=255) cccGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCg > 1:101498/1‑90 (MQ=255) ccATCGCCTTCTGGAAAAGCTAAATCCCTCCCAGGGGAATGTGGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTAc < 2:344059/89‑1 (MQ=255) acccccTTCGGAATAAGCTAAATCCCTTCCAGGGGCATGTGGGTTGTCCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAg < 1:657064/86‑1 (MQ=255) tCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCCCCAGTAATATCAGCATGGCGCTTGCCGTACAGa > 2:614353/1‑90 (MQ=255) tCGCCTTCTGGATAAGCTAAATCCCTCCCGGGGGCATGTGGGTTGTGCCTTCCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGa < 2:633930/90‑1 (MQ=255) cTGGATAAGCTGAACCCCTCCCAGCGCCTTGTGGGTTTGCCCTTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAg < 2:417012/90‑1 (MQ=255) gtaTAAGCTGACTCCTTGCGAGAGGGTTGTGGGTTGGGCCTTCCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAggg < 2:745632/88‑1 (MQ=255) aaTACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATaaa > 2:127700/1‑90 (MQ=255) ccggCGGCATGTGGTTTGCGCCTTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCaa < 2:101498/87‑1 (MQ=255) ccggCGGCATGTAGTTTTTGCCGTTCCCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCaa < 1:431204/87‑1 (MQ=255) | AACCACAAGGGTAACAAACTCAGCACCCCAGGAACCCATTAGCACCCGCATCGCCTTCTGGATAAGCTGAATACCTTCCAGCGGCATGTAGGTTGTGCCGTTACCCGCCAGTAATATCAGCATGGCGCTTGCCGTACAGATGACCAGGGTGTCGATAAAAATGCCAA > NZ_CP009273/6892‑7058 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |