Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	859,587	G→A	A164T (GCA→ACA)	fsa →	fructose‑6‑phosphate aldolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	859,587	0	G	A	100.0%	102.9 / NA	27	A164T (GCA→ACA)	fsa	fructose‑6‑phosphate aldolase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (12/15);  total (12/15)

TGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCAC  >  NZ_CP009273/859505‑859671                                                                                   |                                                                                     tGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAg                                                                               >  2:84049/1‑90 (MQ=255)        ggCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCaaa                                                                        <  2:805523/90‑1 (MQ=255)        ggCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCaaa                                                                        <  1:542823/90‑1 (MQ=255)               gCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCcgc                                                                 >  2:789993/1‑90 (MQ=255)                 ggCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGa                                                                                <  2:174656/73‑1 (MQ=255)                 ggCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGa                                                                                >  1:174656/1‑73 (MQ=255)                        aGACTGTGACCGACTTACCCCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGgcgc                                                        <  2:413178/90‑1 (MQ=255)                             gtgACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGAc                                                   <  1:453308/90‑1 (MQ=255)                                aCCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGc                                                <  1:436509/90‑1 (MQ=255)                                      ttACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTg                                          <  1:338285/90‑1 (MQ=255)                                             aGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGAt                                   >  2:461376/1‑90 (MQ=255)                                              gTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCa                                                             >  1:239896/1‑63 (MQ=255)                                              gTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCa                                                             <  2:239896/63‑1 (MQ=255)                                                  ttGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGt                                                                              <  1:528482/42‑1 (MQ=255)                                                  ttGAAAATGCATGCGCCGCAGGCGAAAGTGCTGACAGCGAGt                                                                              >  2:528482/1‑42 (MQ=255)                                                        atgcatgcGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAAtt                        <  1:37171/90‑1 (MQ=255)                                                        atgcatgcGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAAtt                        >  2:170636/1‑90 (MQ=255)                                                           catgcGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTAct                     >  2:401833/1‑90 (MQ=255)                                                           catgcGCCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTAct                     >  2:653169/1‑90 (MQ=255)                                                              gcgcCGCAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTg                  >  2:177742/1‑90 (MQ=255)                                                              gcgcCGAAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTg                  >  1:669104/1‑90 (MQ=255)                                                                   gcAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACt             >  1:222394/1‑90 (MQ=255)                                                                    cAGGCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTg            <  2:586382/90‑1 (MQ=255)                                                                       gCGAAAGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGAt         <  2:222394/90‑1 (MQ=255)                                                                            aGTGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGc    <  1:461376/90‑1 (MQ=255)                                                                              tGCTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGcac  <  1:177742/90‑1 (MQ=255)                                                                                cTGACAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGcac  <  2:862925/88‑1 (MQ=255)                                                                                   |                                                                                     TGCTCAGGGCGGTAGCGGCATTCAGACTGTGACCGACTTACACCAGTTATTGAAAATGCATGCGCCGCAGGCGAAAGTGCTGGCAGCGAGTTTCAAAACCCCGCGTCAGGCGCTGGACTGCTTACTGGCAGGATGTGAATCAATTACTCTGCCACTGGATGTGGCAC  >  NZ_CP009273/859505‑859671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑