| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 937,171 | 0 | A | G | 62.5% | 0.5 / 11.9 | 16 | T253A (ACT→GCT) | dmsA | dimethylsulfoxide reductase subunit A |
| Reads supporting (aligned to +/- strand): ref base A (1/5); new base G (10/0); total (11/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.37e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.47e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCC > NZ_CP009273/937087‑937251 | aGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTAc < 1:112772/90‑1 (MQ=255) gTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGGGGCTTTCTATCt > 2:475971/1‑90 (MQ=255) atCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTATTATCTTTAAAAg > 1:255068/1‑90 (MQ=255) aGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAg < 2:296305/90‑1 (MQ=255) tAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGACTTACTATCTTTAAAAGGGACGCCAGaa > 2:321711/1‑90 (MQ=255) gTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGGGGGTTACTTTTTTTAAACAGCACCCCCGAAAACTAAt > 1:348291/1‑90 (MQ=255) caggTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCg < 2:300320/87‑1 (MQ=255) ggTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGatc < 2:195384/90‑1 (MQ=255) gTAATAACCCTGGCGAAACGCGAATGAGTGGCGGGGGGGTGGGTTAATATCTTGAAAAGGCACGCCCGAAAACTAATGCCCGCGTGagca > 1:357947/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGGGGGGGGGCTTTCTATCTTGAACAGGGACGCCAGAAAACTAATTCCCGCATTAGCATCATCGATcc > 2:188991/1‑90 (MQ=255) tGGCGAAACGCGAATGAGTGGCGGAGGGGGGGCTTTCTATCTTTAAAAAGGACGCCCAAAAACTTATGCCCCCCTGATTATCATTGGTcc > 2:154636/1‑90 (MQ=255) aaaCGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCt < 1:321711/90‑1 (MQ=255) aCGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGCCTGATCATCATCGATCCCCGCtat > 2:596100/1‑90 (MQ=255) aCGCGAATGAGTGGCGGTGGGGGGGCTTACTATCTTTAAAAGGGACGCCCGAAAACTAATGCCCGCATGATCATCATCGATCCGCGCtct > 1:328499/1‑88 (MQ=255) ggTGGGGGGGCTTTCTATCTTTTACAAGCACGCCAGAAATATAATGCCCGCAAGATCATCATCGGTCCGCGCGATACCCACACCCGGGcc > 1:281181/1‑90 (MQ=255) ggTGGGGGGGCTTTCTATCTTTAACAGGCACGCCAGAAAACTAATGCCCGCATGATCATCATCGATCCCCGCTATACCGACACCCGGGcc > 1:310366/1‑90 (MQ=255) | AGCCCGTCGGATATCGAAAACAGTAAGCTGGTAGTGCTGTTTGGTAATAACCCTGGCGAAACGCGAATGAGTGGCGGTGGGGTGACTTACTATCTTGAACAGGCACGCCAGAAATCTAATGCCCGCATGATCATCATCGATCCGCGCTATACCGACACCGGTGCC > NZ_CP009273/937087‑937251 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |