| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,152,727 | 0 | T | G | 56.2% | 12.2 / 18.8 | 16 | G165G (GGT→GGG) | ycfH | metal‑dependent hydrolase |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (9/0); total (9/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.74e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.80e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152640‑1152816 | cGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTaa < 2:375539/90‑1 (MQ=255) aaTTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGAtct < 2:506737/90‑1 (MQ=255) gcgcGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGAtt < 2:449447/90‑1 (MQ=255) cgcgAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGAttt < 2:408595/90‑1 (MQ=255) cGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGGGAAATAATGGGTTTCGGGTTTTAAATTTCCTTTTTCg > 1:378694/1‑90 (MQ=255) aggTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATtgt < 1:620033/89‑1 (MQ=255) gCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCt < 2:517212/90‑1 (MQ=255) acacTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATAATTGATCTCGGGTTTTTTATCTCCTTTTTCCGCCTTGTGGCCCTccctct > 2:620033/1‑86 (MQ=255) cTGTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGGTCTCGGGTTTTTTATCTCCTTTTTCGGCCTTTTGACCTTCCCTAATGc > 2:528907/1‑90 (MQ=255) gTTTTACAGAGGACAGAGAAACGGCGGGGAAAATACTGGATCTCGGATTTTAAATCTCCTTTTTCCGCGTTTTGGACCTCCGTAATgggg > 2:393754/1‑90 (MQ=255) ttACAGAGGACAGAGAAACGGCGGGGGAAATAATTGGTCTCGGGTTTTATATGTCCTTTTTTTGCATTTTGGCCCTTCCTAAGGCggggg > 1:297119/1‑87 (MQ=255) ttACAGAGGACAGAGAAACGGCGGGGAAATTATTTTGGCTTGGATTTTACATTTCCTTTTTCCGCCTTTTGACCTTCCGGAAAGCggggg > 2:301270/1‑87 (MQ=255) ggACAGAGAAACGGCGGGGAAATTAATTGATCTCGGGTTTTTTATTTCCTTTTTCCGCGTTTTGGCCTTTCCGAATGCGGGGGAACTgcc > 1:343504/1‑89 (MQ=255) ggACAGAGAAACGGCGGGGAAAATACTTGGTCTCGGGTTTTTTATCTCCCTTTTCCGCCTTTTGGCCTTCCCTAATGCGGGGCAACTgcg > 2:374741/1‑90 (MQ=255) gagaAACGGCGGGGAAAATACTTGATTTCGGACTTTACATCTCCCTTTTCGGCAATGTGACCTTCCCTAATGCGGAGGAACGGCCCGGtg > 1:174013/1‑90 (MQ=255) agaAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGAtgc < 2:133151/90‑1 (MQ=255) tAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCCGAGCAACTGCGCGATGCTGCGCGTTATGt < 1:588431/90‑1 (MQ=255) | CGATACGCTGGCAATTCTGCGCGAAGAAAAAGTGACGGATTGCGGTGGCGTACTACACTGTTTTACAGAGGACAGAGAAACGGCGGGTAAATTACTGGATCTCGGATTTTACATCTCCTTTTCCGGCATTGTGACCTTCCGTAATGCGGAGCAACTGCGCGATGCTGCGCGTTATGT > NZ_CP009273/1152640‑1152816 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |