| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,377,967 | 0 | C | A | 60.0% | ‑0.3 / 13.6 | 10 | L84F (TTG→TTT) | ycjW | LacI family DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base C (4/0); new base A (0/6); total (4/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.76e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.23e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAA > NZ_CP009273/1377905‑1378041 | ttGTGCGACTTTTGTAATATCCCGCCGAACCCTTCTTCTCCAGCTTGGGCGGTGATGGCATGAAAAACTTCAGAAAAAACCGGATTcccc < 2:287202/90‑1 (MQ=255) tgaaaTACCACGTAAAAACTTTTTTCTTCAGTTTGGGGGGTGGGGGCATAAAAAACTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTg < 1:475897/87‑1 (MQ=255) tCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAAc > 2:475897/1‑90 (MQ=255) ccccGTCAAACCTTTTTTTTTCACTTTGGGGGGTGAGGGAATGAAAAACTTCAGAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAAc < 2:653411/87‑1 (MQ=255) cACGTCAAAACCTTCTTCTTCAGTTTGGGCGGTGAGGGCATGAAAAACTTAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACg < 1:98602/90‑1 (MQ=255) aCGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACgg > 2:98602/1‑90 (MQ=255) aaaCCTTCTTTTTCAGCTTTGGGGGTGATGGAAGAAAAACCTAAAGAAAAAAAGGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGaa < 1:31513/90‑1 (MQ=255) ttcttcttcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAc > 2:11490/1‑90 (MQ=255) ttcttcttcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAc > 2:469520/1‑90 (MQ=255) ggCGGTGAGGGCATGAAAAACTCAAAAAAAAAAGGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaa < 1:456835/90‑1 (MQ=255) | TTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAA > NZ_CP009273/1377905‑1378041 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |