| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,377,991 | 0 | A | C | 61.5% | 4.8 / 10.7 | 13 | G76G (GGT→GGG) | ycjW | LacI family DNA‑binding transcriptional regulator |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/8); total (5/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 7.77e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.13e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTT > NZ_CP009273/1377905‑1378074 | ttGTGCGACTTTTGTAATATCCCGCCGAACCCTTCTTCTCCAGCTTGGGCGGTGATGGCATGAAAAACTTCAGAAAAAACCGGATTcccc < 2:287202/90‑1 (MQ=255) tgaaaTACCACGTAAAAACTTTTTTCTTCAGTTTGGGGGGTGGGGGCATAAAAAACTTAAGAAAAAAACGGATTCCCCGCCGTAGTTTTg < 1:475897/87‑1 (MQ=255) tCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAAc > 2:475897/1‑90 (MQ=255) ccccGTCAAACCTTTTTTTTTCACTTTGGGGGGTGAGGGAATGAAAAACTTCAGAAAAAACCGGATTCCCCGCCGTAGTTTTGGTCGAAc < 2:653411/87‑1 (MQ=255) cACGTCAAAACCTTCTTCTTCAGTTTGGGCGGTGAGGGCATGAAAAACTTAAAAAAAAAACGGATTCCCCGCCGTAGTTTTGGTCGAACg < 1:98602/90‑1 (MQ=255) aCGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACgg > 2:98602/1‑90 (MQ=255) aaaCCTTCTTTTTCAGCTTTGGGGGTGATGGAAGAAAAACCTAAAGAAAAAAAGGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGaa < 1:31513/90‑1 (MQ=255) ttcttcttcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAc > 2:11490/1‑90 (MQ=255) ttcttcttcAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCAc > 2:469520/1‑90 (MQ=255) ggCGGTGAGGGCATGAAAAACTCAAAAAAAAAAGGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCaaa < 1:456835/90‑1 (MQ=255) gaAAACCGAATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAg < 1:119981/90‑1 (MQ=255) aaaCGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCtt > 1:521877/1‑90 (MQ=255) cgtaTTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGtt < 2:501412/87‑1 (MQ=255) cGGATTCCCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGtt < 1:546696/90‑1 (MQ=255) | TTGTGCGACGTTTGTAATATCACGTCGAAACCTTCTTCTTCAGCTTTGGCGGTGATGGCATGCAAAACTTCAGAGAAAAACGGATTACCCGCCGTAGTTTTGGTCGAACGGGTAGAAATCACCATAATGGCATCAAAACCCGAAGAGGTCAGCGCGCGGGCCAGCTTGTT > NZ_CP009273/1377905‑1378074 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |