| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,622,316 | 0 | T | G | 57.1% | 5.8 / 11.2 | 14 | G181G (GGT→GGG) | ydfG | bifunctional NADP‑dependent 3‑hydroxy acid dehydrogenase/3‑hydroxypropionate dehydrogenase YdfG |
| Reads supporting (aligned to +/- strand): ref base T (3/3); new base G (8/0); total (11/3) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.49e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 3.20e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAG > NZ_CP009273/1622234‑1622404 | ttCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCg < 1:166107/90‑1 (MQ=255) cTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTc < 1:629315/90‑1 (MQ=255) tGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTcc < 2:612183/90‑1 (MQ=255) gAATCTGCGTACGGATCTGCATGGTACGGCGGGGGGGGGTACCGGCAACGAACCGGGGCTGGGGGGGGGTAGCGAGGTTTTCAATggtcg > 1:357519/1‑86 (MQ=255) aaTCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGGGGGGGGGACCGAGGTTTCCAATGTCCGc > 2:629315/1‑90 (MQ=255) aaTCTGCGTACGGATCTGCATGGTACGGCGGGGCGCGTCACCCACAACCAACCGGGGCGGGGGGGGGGGACCGAGGTTTCCCATGTcccc > 1:181854/1‑88 (MQ=255) gTACGGCGGTGCGCGTCACCGACATCGAACCGGGGCTGGTGGGGGGGACCGAGTTTTTCAATGTCCGCTTTAAAAGCGGTGACGGGAAAg > 1:301013/1‑90 (MQ=255) gTGCGCGTCACCCACATCGAACCGGGGCTGGTGGGGGGGACCGAGGTTTCCAATGTCCGCTTTAAAAGCGGTGGCGGGAAAGCAGaaaaa > 1:378823/1‑90 (MQ=255) gcgcGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGGAAAGCAGAAAAAAc > 2:389996/1‑90 (MQ=255) gcgcGTCACCGACATCGAACCGGGTCTGGTGGGGGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAAc > 2:537921/1‑90 (MQ=255) gcgcGTCACCGACATCGAACCGGGTCTGGTGGGGGGTACCGAGGTTTCCAATGTCCGCTTTAAAGGCGATGACGGGAAAGCAGAAAAAAc > 2:283553/1‑90 (MQ=255) gcgcGTCACCCACATCGAAACGGGGCTGGGGGGGGGTACCGAGGTTTTCAATGTCCCCTTTAAAGGCGGTGACGGTAAAGCAGAAAAAAc > 1:550871/1‑90 (MQ=255) cACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCa > 1:356917/1‑90 (MQ=255) tggtggGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGc > 1:14440/1‑90 (MQ=255) gtggtACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCagaag > 1:447831/1‑90 (MQ=255) gtggtACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCagaag > 1:449436/1‑90 (MQ=255) | TTCGTCAGTTTAGCCTGAATCTGCGTACGGATCTGCATGGTACGGCGGTGCGCGTCACCGACATCGAACCGGGTCTGGTGGGTGGTACCGAGTTTTCCAATGTCCGCTTTAAAGGCGATGACGGTAAAGCAGAAAAAACCTATCAAAATACCGTTGCATTGACGCCAGAAG > NZ_CP009273/1622234‑1622404 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |