| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,816,473 | 0 | A | C | 66.7% | 3.4 / 10.7 | 15 | V14G (GTA→GGA) | osmE | osmotically‑inducible lipoprotein OsmE |
| Reads supporting (aligned to +/- strand): ref base A (5/0); new base C (0/10); total (5/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.27e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GGCTGTACAAACTGGTCTTT‑GGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATAC > NZ_CP009273/1816413‑1816558 | ggCTGTACAAACTGGTCTTT‑GGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCata > 2:585345/1‑90 (MQ=255) tGTACAAACTGGTCTTT‑GGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTc > 2:80962/1‑90 (MQ=255) taaaaaCGGGTTTTT‑GGTCCGATAAAAAGCCGTACACCCCGCCAGCATGGTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCtt < 2:390288/87‑1 (MQ=255) aaaaaCTGGTTTTTGGGTACGTTAAAAACCCGTACACCCCCCCAGCATGGTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCtt < 2:607413/88‑1 (MQ=255) gggTCTTT‑GGTACGTTCATAAGCCGTAAACCCCGCCAGCATGGTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCata < 2:406131/89‑1 (MQ=255) aCGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCctct > 1:549208/1‑90 (MQ=255) ccGTACACCCCGCCAGCATGTTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGc < 2:113641/90‑1 (MQ=255) gTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCgt > 2:270039/1‑90 (MQ=255) acacCCCCCCAGCATGTTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCgtgt < 2:312918/90‑1 (MQ=255) ccccaccaTGTTAAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCGTGTTAGATa < 2:517606/88‑1 (MQ=255) cGCCAGCTTGTTTAATCCCGCCGCTGCCCTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCGTGTTAGATa < 2:68000/90‑1 (MQ=255) ccAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAg > 1:315229/1‑90 (MQ=255) aGCATGTTTAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGcc < 2:512587/90‑1 (MQ=255) ttaaaTCCCGCCGCTGCACTCAAAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAAt < 1:291420/87‑1 (MQ=255) tAATCCCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATAc < 1:270039/90‑1 (MQ=255) | GGCTGTACAAACTGGTCTTT‑GGTACGATCATAAGCCGTACAACCCGCCAGCATGGTTAATACCGCCGCTGCACTCAGAATTCCTGCCATATTCTTGTTCATATTCCGTCCTCTTGTTTATCAGCGTGTTAGATAAGCCTGGAATAC > NZ_CP009273/1816413‑1816558 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |