| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,247,828 | 0 | C | A | 55.6% | 9.1 / 21.5 | 18 | V280F (GTT→TTT) | rihB | ribosylpyrimidine nucleosidase |
| Reads supporting (aligned to +/- strand): ref base C (2/6); new base A (10/0); total (12/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.51e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 6.74e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATC > NZ_CP009273/2247747‑2247915 | gcacgcacTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGGTTTCCCAGAACGCCCCGCt > 1:184715/1‑90 (MQ=255) cTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTAc < 2:572237/90‑1 (MQ=255) aaTCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAAAAAGCCCCGCCCGTCGCCGACGGGACGc > 2:553243/1‑90 (MQ=255) aaTCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAAAAAGCCCAGCTCGTCGCAGACGGGACGc > 2:227392/1‑90 (MQ=255) aaTCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCCTTCCCAAAAAACCCCGCCCGTCGCCGACGGGACGc > 1:371055/1‑90 (MQ=255) aaTCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCCTTCCCAAAAAACCCCCCCCCTCGCCGACGGGACGc > 1:567060/1‑90 (MQ=255) cAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAg < 1:252987/90‑1 (MQ=255) aaCCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCaa < 1:557381/90‑1 (MQ=255) aGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCCGCTCGTCGCAGACGGGACGCCCCTAGCAAGGGc > 2:4249/1‑90 (MQ=255) gTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAAAAAGCCCCGCTCGTCGCGAACGGGACGCCCACATCAAGGGCCCCTg > 2:168220/1‑90 (MQ=255) tGATGCCGACTTTGGTATTGGCGGGCTTGGCAAGAAAACCCAACCCGTCGCCGAAGGTACGCCCATAGCGAGGGGCCCGGTTGGCGGccc > 1:228199/1‑89 (MQ=255) tGATGCCGACTTTGGTATTGGCGGGCTTGCCCAAAAAGCCCCGCCCGTCGGCGACGGGAAGCCCCTAGGAAGGGGCCCTGTTGACGGccc > 1:348654/1‑89 (MQ=255) tGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAAAGCCCAGCTCCTCGCCGACGGGACGCCCCTAGAAAGGGCCACTGTTGACGGccc > 2:143743/1‑89 (MQ=255) gATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAAAGCCCCGCTCCTCGCCGACGGGACGCCCATAGCAAGGGGCACTCTTGACGTcccc > 2:47164/1‑88 (MQ=255) gACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGAc < 1:281681/90‑1 (MQ=255) cTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGt < 2:348654/90‑1 (MQ=255) cTTTGGTATTGGCGGGCTTGCCAAGAAAGCCCAGCTCGTCGCAGACGGGACGCCCCTAGCAAGGGCCACTGTTGGCGTCCCCTTCGACGt > 2:281681/1‑90 (MQ=255) aaCGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATc < 2:637589/90‑1 (MQ=255) | GCACGCACTCTTCGACTAATCCCCAGAACCAGTCTGTATCAATAGTGATGCCGACTTTGGTATTGGCGGGCTTGCCAAGAACGCCCAGCTCGTCGCAGACGGTACGCCCATAGCAAGGGCCACTGTTGACGTCCACTTCGACGTACATCTCCTGGGTTTTAATGCCATC > NZ_CP009273/2247747‑2247915 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |