| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,290,243 | 0 | T | G | 58.3% | 5.1 / 10.7 | 12 | L87F (TTA→TTC) | ccmB | heme exporter protein CcmB |
| Reads supporting (aligned to +/- strand): ref base T (0/5); new base G (7/0); total (7/5) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.26e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.38e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGC > NZ_CP009273/2290157‑2290330 | gCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCCAAGGGAAc > 2:63204/1‑90 (MQ=255) aGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAAt < 1:440542/90‑1 (MQ=255) tGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGGAAAGGCAACAAAATCAATTGtt > 2:271563/1‑90 (MQ=255) ggCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGGGAGGGGAACGGCCACAACAACAATTTTTTCAGAccg > 1:53624/1‑88 (MQ=255) aGTGCGCCATCACCTTCGCCCGCACAACGGCGGGCAAGGGGAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCAc > 2:585606/1‑90 (MQ=255) gCACAACGGCGGGCAAGGGGAACGGCAACAAAATCAATTGTTCAAGACTGCCGGCCTGCAAAACGTCACGGAACAGTCGTTCCAGCGccc > 1:322108/1‑89 (MQ=255) cacaACGGCGGGGAAGGGGAACGGCAACAACATCAATTGGTTAAAACCGCCGGCCTGCAAATCGTCACGGAACAGGCGTTTCAGCGcccg > 1:161323/1‑88 (MQ=255) aaCGGCGGGCAAGGGTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCaa < 1:271563/90‑1 (MQ=255) aaCGGCGGGCAAGGGTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCaa < 2:161323/90‑1 (MQ=255) aaCGGCGGGCAAGGGTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCaa < 2:273835/90‑1 (MQ=255) aCGGCGGGCAAGGGGAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAg > 2:522078/1‑90 (MQ=255) ggTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAagcagc < 2:235290/90‑1 (MQ=255) | GCTACCAGTGGCGAAAGGATGAGTAACGGCAGACCGGTTACCATCCAGTGCGCCATCACCTTCGCCAGCACAACGGCGGGCAAGGGTAACGGCAACAACATCAATTGTTCAAGACTGCCGTCCTGCAAATCGTCACGGAACAGTCGTTCCAGCGCCAGCAAGGATGAAAGCAGC > NZ_CP009273/2290157‑2290330 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |