| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,558,321 | 0 | T | G | 73.3% | 1.1 / 14.8 | 30 | T6P (ACC→CCC) | mdtM | multidrug efflux MFS transporter MdtM |
| Reads supporting (aligned to +/- strand): ref base T (2/6); new base G (22/0); total (24/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.72e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.36e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGT‑CCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAA > NZ_CP009273/4558234‑4558398 | cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaa > 1:241488/1‑90 (MQ=255)cAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaa > 1:665491/1‑90 (MQ=255) ggCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaa > 1:249451/1‑90 (MQ=255) ggATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaaaaaa > 2:42116/1‑89 (MQ=255) gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaaaaaag > 2:481338/1‑88 (MQ=255) aTCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaaaaaaag > 1:318296/1‑87 (MQ=255) gATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCa > 1:511983/1‑90 (MQ=255) gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaaca < 1:269560/90‑1 (MQ=255) gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGCATaaaa > 2:23513/1‑88 (MQ=255) gTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGT‑CCGgaga < 2:355456/90‑1 (MQ=255) acagtACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGT‑CCGgaga < 2:218863/86‑1 (MQ=255) aTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGGATAAAAAAAAACTCCTTTT‑CCGGAGAACCCCCACgg > 2:103645/1‑90 (MQ=255) aTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAAAAAAAAAATCCTTTT‑CCGGAGAACCCCAACgg > 2:599376/1‑90 (MQ=255) cAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAACTCCTTTT‑CCGGAGAACCCCCAAgggg > 2:77443/1‑90 (MQ=255) aaCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAAGGCATAAAAAAAAACACCCTTT‑CCGGGGAACCCCCACGGGGa > 1:610150/1‑90 (MQ=255) aCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAACGCGGGCTAAAAAAAAACTCCTTGT‑CCGGGGAACCCCCAAggggga > 2:148700/1‑88 (MQ=255) aCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGAGGGATAAAAAAAAAAACCTTTT‑TCGGGGAAACCCCACggggga > 1:318289/1‑88 (MQ=255) aCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAATGGGATAAAAAAAAACACCTTTTCCCGGGGAACCCCACCgctgt > 1:218863/1‑86 (MQ=255) aCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAAGGCATAAAAAAAAAACCCTTTT‑CCGGGGAACCCCCACggggga > 1:80602/1‑88 (MQ=255) cATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAAGGGAAAAAAAAAAAAACCCTTT‑CCCGGGAAACCCCACGGGGaaaaag > 1:258668/1‑88 (MQ=255) aTCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAGGGGATAAAAAAAAAAACCCTGT‑CCGGGGAACCCCAACGGGGGAAAAGa > 1:527560/1‑90 (MQ=255) aCAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGT‑CCGGAGAACCCCAACGGGGAAAACGAAATTCAATAc < 1:639158/90‑1 (MQ=255) aGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGT‑CCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCg < 2:560873/90‑1 (MQ=255) aGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCAAAAAAAAAAAATCCTTTT‑CCGGAGAACCCCCACGGGGGAAAAGAAAATCAATAccc > 2:545031/1‑89 (MQ=255) aGCGTGGCGGCATGGCGGGGAAAAAAAAAGGGGAAAACAAAAAACTCCCTTT‑CCGGAGGAACCCAACGGGGGAAAAGAAAATCAAAACCg > 1:428153/1‑90 (MQ=255) gCGTGGCGGCATGGCGGGTAAAAAAAAGGGGCATAAAAAACAACTCCTTTT‑CCGGGGAACCCCAACGGGGGAAAAGAAAATCAAAACCGt > 2:98257/1‑90 (MQ=255) gCGTGGCGGCATGGCGGGGAAAAAAAAGGGGCATAACAAACAACTCCCTGT‑CCGGAGAACCCCAACGGGGAAAAAGAAAATTAATAcccg > 2:197154/1‑88 (MQ=255) gcggcATGGCGGGTAAAAAAAAATGGGATAACAAAAAAACCCCTTT‑CCGGGGAACCCCAACGGGGGAAAAGAAAATAAATACCGTCggga > 1:67559/1‑88 (MQ=255) gcggcATGGCGGGGAAAAAAAAGGGGGATAAAAAAAAACCCCTTTT‑CCGGGGAACCCCCACGGGGAAAAAGAAATTCAAAACCGTCggga > 1:192022/1‑88 (MQ=255) cggcATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGT‑CCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa < 2:428153/90‑1 (MQ=255) | CAGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGT‑CCGGAGAACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAA > NZ_CP009273/4558234‑4558398 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |