Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,903	T→G	D27E (GAT→GAG)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,903	0	T	G	100.0%	64.1 / NA	20	D27E (GAT→GAG)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (8/12);  total (8/12)

CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49828‑49982                                                                            |                                                                                cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTaa                                                                   >  2:198686/1‑90 (MQ=255)   gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAAc                                                                 <  1:647451/90‑1 (MQ=255)   gTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAAc                                                                 >  1:587933/1‑90 (MQ=255)        aTTGCGGCTTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAac                                                            <  2:338408/90‑1 (MQ=255)            cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt                                                        <  1:60154/90‑1 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGcc                                                                           <  1:128785/70‑1 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGcc                                                                           >  2:128785/1‑70 (MQ=255)                gTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAac                                                            <  2:408948/82‑1 (MQ=255)                gTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAac                                                            >  1:408948/1‑82 (MQ=255)                  tAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTtaaata                                                  <  1:393437/90‑1 (MQ=255)                         aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                           <  2:486907/90‑1 (MQ=255)                         aGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGt                                           <  1:366978/90‑1 (MQ=255)                             cgcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGAtt                                       <  2:131832/90‑1 (MQ=255)                               cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt                                                        >  1:392972/1‑71 (MQ=255)                               cgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCt                                                        <  2:392972/71‑1 (MQ=255)                                        cATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCa                            <  1:260081/90‑1 (MQ=255)                                                        ccGTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACTCCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatcaatc            <  2:170788/90‑1 (MQ=255)                                                          gTGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCgg          >  1:293486/1‑90 (MQ=255)                                                           tGGAACCTGCCTGCCGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgt         >  1:172391/1‑90 (MQ=255)                                                                  tgcctgccGAGCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGt  >  1:192087/1‑90 (MQ=255)                                                                            |                                                                                CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTCGTCCGT  >  NZ_CP009273/49828‑49982

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑