| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 50,280 | T→G | F153C (TTT→TGT) | folA → | type 3 dihydrofolate reductase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 50,280 | 0 | T | G | 100.0% | 54.4 / NA | 16 | F153C (TTT→TGT) | folA | type 3 dihydrofolate reductase |
| Reads supporting (aligned to +/- strand): ref base T (0/0); new base G (7/9); total (7/9) | |||||||||||
TTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTC‑TTATCCGGCCTTCCTA > NZ_CP009273/50194‑50366 | tttCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTGTga < 2:374073/90‑1 (MQ=255) ccGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTGTGAGAtt > 1:177570/1‑90 (MQ=255) ttCAGCGAATTCCACGATGCTGATGTGCAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAAATTTCg > 2:341550/1‑90 (MQ=255) gCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAGCGGCGGGAATTTTGTATAGAATTTACGGCTa > 1:481799/1‑90 (MQ=255) cgcAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTc < 1:374212/90‑1 (MQ=255) gcAGAACTCTCACAGCTATTGCTGTGAGATTCTGGAGCGGCGGTAATTTTTTATAGAATTTTCGGCTAGCGCCGGGTGGGGGGCCGGGcg > 1:341433/1‑90 (MQ=255) cacaGCTATTGCTGTGAGATTCTGGAGCGGCGGTAAtt > 1:333323/1‑38 (MQ=255) cacaGCTATTGCTGTGAGATTCTGGAGCGGCGGTAAtt < 2:333323/38‑1 (MQ=255) gCTATTGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTC‑TTATCCGGcc < 2:454134/90‑1 (MQ=255) ttGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTC‑TTATCCGGCCTTcc < 1:240275/90‑1 (MQ=255) ttGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTC‑TTATCCGGCCTTcc < 2:177570/90‑1 (MQ=255) ttGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTC‑TTATCCGGCCTTcc < 2:481799/90‑1 (MQ=255) ttGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTC‑TTATCCGGCCTTcc < 2:491453/90‑1 (MQ=255) ttGCTGTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTC‑TTATCCGGCCTTcc < 2:96964/90‑1 (MQ=255) ttGCTGTGAGATTCTGGAGCGGCGGGAAATTTGTATAGAAATTACGGCTAACGCCGGGTTCGGCGCCCGGCGCGTCTTTTTCCGCCCTTc > 2:89060/1‑90 (MQ=255) gCTGTGAGATTCTGGAGCGGCGGGAATTTTGTATAGAATTTTAGGGTAACCCCGGGTTCCACGCCGGCCGCCGC‑TTATCCTGGCTTTCtt > 2:611337/1‑89 (MQ=255) | TTTCCCGGATTACGAGCCGGATGACTGGGAATCGGTATTCAGCGAATTCCACGATGCTGATGCGCAGAACTCTCACAGCTATTGCTTTGAGATTCTGGAGCGGCGGTAATTTTGTATAGAATTTACGGCTAGCGCCGGATGCGACGCCGGTCGCGTC‑TTATCCGGCCTTCCTA > NZ_CP009273/50194‑50366 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |