Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,613,378	T→C	M1A (GTG→GCG) †	marR →	multiple antibiotic resistance transcriptional regulator MarR

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,613,378	0	T	C	100.0%	96.3 / NA	26	M1A (GTG→GCG) †	marR	multiple antibiotic resistance transcriptional regulator MarR
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/11);  total (15/11)

GGTCAATTCATTCATTTGACTTATACTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAA  >  NZ_CP009273/1613299‑1613451                                                                                |                                                                          ggTCAATTCATTCATTTGACTTATACTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTAcc                                                                 >  1:195050/1‑90 (MQ=255) ggTCAATTCATTCATTTGACTTATACTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTAcc                                                                 <  2:319205/90‑1 (MQ=255)               tttGACTTATACTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCaa                                                   >  2:299502/1‑90 (MQ=255)                        taCTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAattat                                          <  1:159858/90‑1 (MQ=255)                            tGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAg                                                               <  2:592344/65‑1 (MQ=255)                            tGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAg                                                               >  1:592344/1‑65 (MQ=255)                            tGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCa                                      >  2:573970/1‑90 (MQ=255)                            tGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCa                                      >  2:243544/1‑90 (MQ=255)                                 gggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTggg                                 >  2:197440/1‑90 (MQ=255)                                  ggCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGt                                <  2:45808/90‑1 (MQ=255)                                      atatTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCt                            >  1:301944/1‑90 (MQ=255)                                             ccccTGCAACTAATTACTCGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTggg                                 >  1:296612/1‑78 (MQ=255)                                             ccccTGCAACTAATTACTCGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTggg                                 <  2:296612/78‑1 (MQ=255)                                                cTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCatat                  >  1:123615/1‑90 (MQ=255)                                                cTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCatat                  >  2:216116/1‑90 (MQ=255)                                                cTGCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCatat                  >  2:422026/1‑90 (MQ=255)                                                  gCAACTAATTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATgg                >  1:483665/1‑90 (MQ=255)                                                        aaTTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATc          <  2:63051/90‑1 (MQ=255)                                                        aaTTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATc          <  1:555065/90‑1 (MQ=255)                                                         aTTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCa         <  2:18470/90‑1 (MQ=255)                                                         aTTACTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCa         <  2:520392/90‑1 (MQ=255)                                                            aCTTGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCagaa      >  1:89857/1‑90 (MQ=255)                                                               tGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAgaagaa   <  1:40797/90‑1 (MQ=255)                                                               tGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAgaagaa   >  1:640951/1‑90 (MQ=255)                                                               tGCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAgaagaa   >  1:562783/1‑90 (MQ=255)                                                                gCCAGGGCAACTAATGCGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGaagaaa  <  2:480225/90‑1 (MQ=255)                                                                                |                                                                          GGTCAATTCATTCATTTGACTTATACTTGCCTGGGCAATATTATCCCCTGCAACTAATTACTTGCCAGGGCAACTAATGTGAAAAGTACCAGCGATCTGTTCAATGAAATTATTCCATTGGGTCGCTTAATCCATATGGTTAATCAGAAGAAA  >  NZ_CP009273/1613299‑1613451

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑