| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,058,958 | 0 | G | A | 57.1% | ‑2.7 / 15.4 | 21 | L57L (CTG→TTG) | yihO | MFS transporter |
| Reads supporting (aligned to +/- strand): ref base G (5/4); new base A (0/12); total (5/16) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.19e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.18e-02 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058879‑4059038 | ttAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGGGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATc > 1:597216/1‑90 (MQ=255) gCCAAATTTTTTCCCGCGAGTCGAAAAAAAATCGGGTGAGCATATGGGAAAACGCGGAAAAAACCTTCCCCACAAAAAAGATAATCCCGc < 1:696482/90‑1 (MQ=255) ttcccGCGAGTCGAGAAAAAATCCGGTGAGCATATCGGTAAACGCGGAAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGc < 2:301257/87‑1 (MQ=39) ccGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGc > 1:288773/1‑77 (MQ=255) ccGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGc < 2:288773/77‑1 (MQ=255) cgcgAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGc < 2:311428/90‑1 (MQ=255) gggagtggagtAAAAACCGGGGGAGCATATGGGTAAACGCGGTAAAAACCTCCCCCACAAAAAATATAATCCCGCCATAGTAAGCAGGCa < 2:722253/88‑1 (MQ=255) gcgAGTCGAGAAAAAAGCGGGGGAGCATACCGGAAAACGGGAAAAAAAACTTCCCCCCCAGAAAGATAACCCCGCCATAGTAAGCAGGCa < 1:254070/90‑1 (MQ=255) aaaCCCGGGGAGAATATCGGAAAACGCGAAAAAAAACTCCGCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATc < 2:342624/90‑1 (MQ=255) cGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtat > 1:263965/1‑90 (MQ=255) ggTGAGCATATCGGTAAACGGGGAAAAAACCTCCGCCACAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata < 1:72675/90‑1 (MQ=255) ggTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGtata > 2:290334/1‑90 (MQ=255) attatCGGAAAACGCGAAAAAAATCTCCCCCCCAAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAAttt < 1:157184/88‑1 (MQ=255) tCGGTAACCGCGAAAAAAACCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 1:134066/90‑1 (MQ=255) tCGGAAACCGCGGAAAAAAACTCCCCCCCCAAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTgagg < 2:263965/90‑1 (MQ=255) gaaaaCGCGAAAAAAAACTTCCCCACAAAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGgaga < 2:367772/88‑1 (MQ=255) aaaaacCTCCGCCCCCAAAAAAAAAACCCCGCCATAGTGAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAcc < 2:440592/90‑1 (MQ=255) aaaaacCTCCCCCACAAAAAAAAAAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTAcc < 1:19799/90‑1 (MQ=255) tcccccacaaAAAAAATAACCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCag < 1:389680/86‑1 (MQ=255) tCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga > 1:576250/1‑90 (MQ=255) cccccaccaAAAAAATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCaga < 2:295773/87‑1 (MQ=255) | TTAGGCCCAATATTTTTCCGCGAGTCGAGTAAAAATCCGGTGAGCATATCGGTAAACGCGGTAAAAAACTTCGCCACCAGAAAGATAATCCCGCCATAGTAAGCAGGCATCCCTAATTCATCGGTATAAAATTTGAGGAGATACAGAGTACCGATGCAGA > NZ_CP009273/4058879‑4059038 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |